PXD008830

PXD008830 is an original dataset announced via ProteomeXchange.

Title	RBPome of primary mouse T helper cells
Description	Post-transcriptional gene regulation by mRNA-binding proteins (RBPs) ensures an important layer to control protein outcome. It includes many fundamental processes, ranging from splicing to transport, decay and translation. Although recent technical developments allow the identification of mRNA-binding proteins, the total RBPome of different primary immune cells remains unknown. Here, we performed an RBP capture of the two major primary T helper cell types in mouse: T helper effector-like and regulatory-like (Foxp3+) cells. The two datasets show a high overlap of ~80% to reveal a high confidence common T helper cell RBPome of 240 proteins. 60% of those proteins have not been previously associated with any immune function. Most importantly, 11 RBPs were not previously identified through any mammalian mRNA-capture approach. These novel RBPs include some well-known T cell proteins associated with non-immune functions such as Stat1 and Vav1. Overall our data suggest an underestimated complexity of gene regulatory pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:23:31.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-01 01:54:16	ID requested
1	2021-07-01 08:35:24	announced
⏵ 2	2024-10-22 05:23:31	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: RBP, mRNA interactome capture, immune cells

Matthias Mann
contact affiliation	Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany
contact email	mmann@biochem.mpg.de
lab head
Michael Wierer
contact affiliation	Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany
contact email	wierer@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008830
     1. Label: PRIDE project
     2. Name: RBPome of primary mouse T helper cells