PXD008829

PXD008829 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas aeruginosa PA3904 pull down coupled to LC-MS/MS
Description	pETDUET His-PAAR4 and pETDUET Flag-PAAR4 were transformed into BL21 E. coli DE3 and expressed as described above.  An equal volume of PAO1 retS was grown to an OD600 of 2.0.  E. coli expressing His-PAAR4 or Flag-PAAR4 and Pseudomonas cells were harvested by centrifugation and lysed as described above.  Lysates of both species were combined and His-PAAR4 or Flag-PAAR4 were purified as before.  Eluted sample was mixed with SDS-loading buffer, boiled and run on a 12% SDS-PAGE for 0.6 cm.  After coomassie staining and de-staining, the resulting band was excised and used for LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2018-03-09
AnnouncementXML	Submission_2018-04-16_01:39:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008829
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Brianne Burkinshaw
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-01 01:47:32	ID requested
1	2018-03-09 08:05:16	announced
⏵ 2	2018-04-16 01:39:23	announced	Updated publication reference for PubMed record(s): 29632369.

Burkinshaw BJ, Liang X, Wong M, Le ANH, Lam L, Dong TG, A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol, 3(5):632-640(2018) [pubmed]

curator keyword: Biological

submitter keyword: Pseudomonas aeruginosa

Tao Dong
contact affiliation	Ecosystem and Public Health, Faculty of Veterinary Medicine; Biochemistry and Molecular Biology, Cumming School of Medicine; Snyder Institute for Chronic Diseases, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB, T2N4Z6, Canada
contact email	tdong@ucalgary.ca
lab head
Brianne Burkinshaw
contact affiliation	University of Calgary
contact email	brianne.burkinsha1@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008829

PRIDE project URI

• PRIDE
  1. PXD008829
     1. Label: PRIDE project
     2. Name: Pseudomonas aeruginosa PA3904 pull down coupled to LC-MS/MS