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PXD008814

PXD008814 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSPD1590_iTRAQ - A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
DescriptionStreptococcus pneumoniae has three main transporters for iron acquisition: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in a piuA/piaA/pitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present work, using spd1590-deleted and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:00:31.152.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXin Yu Miao
SpeciesList scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: 373153;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-31 01:15:53ID requested
12018-08-08 09:08:18announced
22024-10-22 04:00:32announced2024-10-22: Updated project metadata.
Publication List
10.3389/fmicb.2018.01624;
Miao X, He J, Zhang L, Zhao X, Ge R, He QY, Sun X, Contributing to Bacterial Virulence Properties. Front Microbiol, 9():1624(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Streptococcus pneumoniae D39, iTRAQ labeling, Orbitrap Fusion Lumos
Contact List
Xuesong Sun
contact affiliationKey Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
contact emailtsunxs@jnu.edu.cn
lab head
Xin Yu Miao
contact affiliationJinan University
contact emailmiaoxinyu66@126.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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