PXD008814 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SPD1590_iTRAQ - A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties |
Description | Streptococcus pneumoniae has three main transporters for iron acquisition: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in a piuA/piaA/pitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present work, using spd1590-deleted and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:00:31.152.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xin Yu Miao |
SpeciesList | scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: 373153; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-01-31 01:15:53 | ID requested | |
1 | 2018-08-08 09:08:18 | announced | |
⏵ 2 | 2024-10-22 04:00:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3389/fmicb.2018.01624; |
Miao X, He J, Zhang L, Zhao X, Ge R, He QY, Sun X, Contributing to Bacterial Virulence Properties. Front Microbiol, 9():1624(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Streptococcus pneumoniae D39, iTRAQ labeling, Orbitrap Fusion Lumos |
Contact List
Xuesong Sun |
contact affiliation | Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China |
contact email | tsunxs@jnu.edu.cn |
lab head | |
Xin Yu Miao |
contact affiliation | Jinan University |
contact email | miaoxinyu66@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008814
- Label: PRIDE project
- Name: SPD1590_iTRAQ - A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties