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PXD008807

PXD008807 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLocalised protein expression changes in different regions of human Alzheimer’s brain correlate with disease severity - Motor cortex
DescriptionHuman brain tissue was obtained from the New Zealand Brain Bank from donors with Alzheimer's disease (AD) or age-matched controls (n=9/group). Mean post mortem delays were ~12h. Six distinct brain regions were dissected, Hippocampus, Cingulate Gyrus, Entorhinal Cortex (severely affected), Motor and Sensory Cortex (less affected) and Cerebellum (spared). Protein was extracted from each sample and, each region was analysed independently. For each region, samples were divided into 3 sets of 3 cases and 3 controls and assigned to an iTRAQ 8 plex, alongside two aliquots of a pooled QC sample. Samples were digested, iTRAQ labelled and analysed. For each region, between 3000 and 4200 proteins were compared between AD and control, identifying a series of known and novel protein expression changes associated with the progression of AD.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:00:44.467.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRichard Unwin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
InstrumentQSTAR
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-30 09:00:53ID requested
12019-02-15 14:23:23announced
22024-10-22 04:00:45announced2024-10-22: Updated project metadata.
Publication List
Xu J, Patassini S, Rustogi N, Riba-Garcia I, Hale BD, Phillips AM, Waldvogel H, Haines R, Bradbury P, Stevens A, Faull RLM, Dowsey AW, Cooper GJS, Unwin RD, Regional protein expression in human Alzheimer's brain correlates with disease severity. Commun Biol, 2():43(2019) [pubmed]
10.1038/s42003-018-0254-9;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Human, Dementia, Brain, Motor cortex, Alzheimer’s, iTRAQ
Contact List
Richard David Unwin
contact affiliationDivision of Cardiovascular Science, School of Medical Sciences, Faculty of Biology Medicine and Health, The University of Manchester
contact emailr.unwin@manchester.ac.uk
lab head
Richard Unwin
contact affiliationUniversity of Manchester
contact emailr.unwin@manchester.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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