PXD008806

PXD008806 is an original dataset announced via ProteomeXchange.

Title	Localised protein expression changes in different regions of human Alzheimer’s brain correlate with disease severity - Entorhinal cortec
Description	Human brain tissue was obtained from the New Zealand Brain Bank from donors with Alzheimer's disease (AD) or age-matched controls (n=9/group). Mean post mortem delays were ~12h. Six distinct brain regions were dissected, Hippocampus, Cingulate Gyrus, Entorhinal Cortex (severely affected), Motor and Sensory Cortex (less affected) and Cerebellum (spared). Protein was extracted from each sample and, each region was analysed independently. For each region, samples were divided into 3 sets of 3 cases and 3 controls and assigned to an iTRAQ 8 plex, alongside two aliquots of a pooled QC sample. Samples were digested, iTRAQ labelled and analysed. For each region, between 3000 and 4200 proteins were compared between AD and control, identifying a series of known and novel protein expression changes associated with the progression of AD.
HostingRepository	PRIDE
AnnounceDate	2019-02-15
AnnouncementXML	Submission_2019-02-15_14:06:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Unwin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-30 08:58:44	ID requested
⏵ 1	2019-02-15 14:06:57	announced

Xu J, Patassini S, Rustogi N, Riba-Garcia I, Hale BD, Phillips AM, Waldvogel H, Haines R, Bradbury P, Stevens A, Faull RLM, Dowsey AW, Cooper GJS, Unwin RD, Regional protein expression in human Alzheimer's brain correlates with disease severity. Commun Biol, 2():43(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, Brain, Alzheimer’s, Dementia, iTRAQ, Entorhinal cortex

Richard David Unwin
contact affiliation	Division of Cardiovascular Sciences, School of Medical Science, Faculty of Biology, Medicine and Health, The University of Manchester
contact email	r.unwin@manchester.ac.uk
lab head
Richard Unwin
contact affiliation	University of Manchester
contact email	r.unwin@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008806

PRIDE project URI

• PRIDE
  1. PXD008806
     1. Label: PRIDE project
     2. Name: Localised protein expression changes in different regions of human Alzheimer’s brain correlate with disease severity - Entorhinal cortec