PXD008805

PXD008805 is an original dataset announced via ProteomeXchange.

Title	Asparagine bioavailability governs metastasis in a model of breast cancer
Description	Using a functional model of breast cancer heterogeneity, we previously showed that clonal sub-populations proficient at generating circulating tumour cells were not all equally capable of forming metastases at secondary sites1. A combination of differential expression and focused in vitro and in vivo RNAi screens revealed candidate drivers of metastasis that discriminated metastatic clones. Among these, Asparagine Synthetase (ASNS) expression in a patient’s primary tumour was most strongly correlated with later metastatic relapse. Here, we have shown that asparagine bioavailability strongly influences metastatic potential. Limiting asparagine by Asns knockdown, treatment with L-asparaginase, or dietary asparagine restriction reduced metastasis without impacting growth of the primary tumour, whereas increased dietary asparagine or enforced Asns expression promoted metastatic progression. Altering asparagine availability in vitro strongly influenced invasive potential, and this was correlated with an impact on proteins that promote the epithelial to mesenchymal transition. This provides at least one potential mechanism for how the bioavailability of a single amino acid could regulate metastatic progression.
HostingRepository	PRIDE
AnnounceDate	2018-02-07
AnnouncementXML	Submission_2018-02-07_11:47:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evangelia Papachristou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-30 08:48:03	ID requested
⏵ 1	2018-02-07 11:47:25	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: breast cancer, Asparagine Synthetase, TMT, Q-Exactive

SImon Knott
contact affiliation	Cedars-Sinai Medical Institute, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA
contact email	Simon.Knott@cshs.org
lab head
Evangelia Papachristou
contact affiliation	Senior Scientific Assistant
contact email	Eva.Papachristou@cruk.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008805

PRIDE project URI

• PRIDE
  1. PXD008805
     1. Label: PRIDE project
     2. Name: Asparagine bioavailability governs metastasis in a model of breast cancer