PXD008803 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dynamics of the Escherichia coli proteome in response to the deletion of the Tat pathway |
Description | In Escherichia coli, the Twin-arginine (Tat) secretion system is one of the main routes of the protein export to the periplasm. The Tat pathway secretes a set of proteins with important physiological functions. In our study, we investigated the influence of the deactivation of the Tat pathway on the E. coli cells. We applied a comprehensive and comparative proteomic analysis of the E. coli wild type and tat mutant. This dataset provides mass spectrometry based details on the abundances of proteins in cytoplasmic, periplasmic and membrane fractions. We observed that a tat deletion increases abundances of proteins involved in protein folding, degradation, responses to heat, oxidation, osmolarity, and cold. Moreover, the impairment of E. coli outer membrane resulted in the activation of proteins responsible for cell wall biogenesis. The tat deletion negatively affects the synthesis of iron transporters and imbalances its homeostasis in the cell. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-18 |
AnnouncementXML | Submission_2019-02-18_06:00:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katarzyna Dolata |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-01-30 08:43:31 | ID requested | |
⏵ 1 | 2019-02-18 06:00:40 | announced | |
Publication List
Dolata KM, Montero IG, Miller W, Sievers S, Sura T, Wolff C, Schl, ü, ter R, Riedel K, Robinson C, Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses. Microbiol Res, 218():97-107(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Escherichia coli, Stress responses, Tat pathway |
Contact List
Katarzyna Dolata |
contact affiliation | Institute of Microbiology, Department of Microbial Physiology and Molecular Biology Ernst-Moritz-Arndt-University Greifswald Felix-Hausdorff-Straße 8 17489 Greifswald |
contact email | dolatak@uni-greifswald.de |
lab head | |
Katarzyna Dolata |
contact affiliation | University of Greifswald |
contact email | dolatak@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008803 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008803
- Label: PRIDE project
- Name: Dynamics of the Escherichia coli proteome in response to the deletion of the Tat pathway