PXD008796

PXD008796 is an original dataset announced via ProteomeXchange.

Title	TRANSCRIPTIONAL ACTIVATION BY E2F4 INDEPENDENT OF THE RB FAMILY
Description	E2F transcription factors are central regulators of cell cycle progression and cell fate decisions in mammalian cells. E2F4 is a transcriptional repressor implicated in cell cycle arrest and whose repressive activity depends on its interaction with members of the RB family. E2F4 often represents the predominant E2F activity in cells. Here we show that E2F4 is important for the proliferation and the survival of mouse embryonic stem cells. In these cells, E2F4 acts in part as a transcriptional activator that promotes the expression of cell cycle genes. Importantly, this role for E2F4 is completely independent of the RB family. Accordingly, an unbiased analysis of the E2F4 interactome shows that E2F4 functionally interacts with chromatin regulators associated with gene activation in RB family-mutant cells. Taken together, our findings uncover a non-canonical role for E2F4 that reveal novel insights into the biology of rapidly dividing cell types.
HostingRepository	PRIDE
AnnounceDate	2019-04-26
AnnouncementXML	Submission_2019-07-08_00:20:41.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008796
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Janos Demeter
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Propionamide; Glu->pyro-Glu; Deamidated; Methyl; Biotin; Oxidation; Acetyl; GlyGly; Gln->pyro-Glu
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-30 05:54:00	ID requested
1	2019-04-26 01:34:32	announced
⏵ 2	2019-07-08 00:20:42	announced	Updated publication reference for PubMed record(s): 31270324.

Hsu J, Arand J, Chaikovsky A, Mooney NA, Demeter J, Brison CM, Oliverio R, Vogel H, Rubin SM, Jackson PK, Sage J, E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family. Nat Commun, 10(1):2939(2019) [pubmed]

curator keyword: Biological

submitter keyword: E2F4, RB, cell cycle, embryonic stem cells, p107, p130, pluripotency

Julien Sage
contact affiliation	Department of Pediatrics and Genetics, Stanford University, Stanford, CA 94305, USA
contact email	julsage@stanford.edu
lab head
Janos Demeter
contact affiliation	Stanford University
contact email	jdemeter@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD008796

PRIDE project URI

• PRIDE
  1. PXD008796
     1. Label: PRIDE project
     2. Name: TRANSCRIPTIONAL ACTIVATION BY E2F4 INDEPENDENT OF THE RB FAMILY