The study of peptide secretion, and identifying its control requires the use of ELISAs for measuring their levels pre and post stimulation. In place of ELISA's an LC-MS methdology was developed first to identify the correct peptides generated by the neurons, which could then be used to measure the peptide products by targetted LC-MS analysis. The Extraction was also performed on human hypothalamic tissue obtained from a tissue bank. Samples were analysed by nano LC-MS, and searched using Peaks 8.5.