PXD008792

PXD008792 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis in microdissected renal vessels of hypertensive and normotensive rats
Description	Systemic hypertension has a profound impact on the renal vascular physiology. In order to elucidate the biological pathways and macromolecules deregulated by hypertension renal vessels were obtained by Laser Capture Microdissection (LCM) from Spontaneously Hypertensive Rats (SHR) and age-matched controls (20 weeks). Proteomic analysis was performed aiming to detect early molecular alterations associated with hypertension at the renal vessels before the onset of vascular damage. Proteomic analysis identified 688 proteins, of which 58 were differentially expressed (15 up-regulated and 43 down-regulated in SHR). Many of these proteins are involved in vascular tone regulation by modulating the activity of endothelial Nitric Oxide Synthase (eNOS) (e.g. Xaa-Pro aminopeptidase 1 (XPP1), N(G) N(G)-dimethylarginine dimethylaminohydrolase 1 (DDAH1), Dehydropteridine reductase (DHPR)) or in blood pressure control by regulating the renin-angiotensin system (e.g. Glutamyl aminopeptidase/Aminopeptidase A (AMPE), Aminopeptidase N (AMPN)). Moreover, pathway enrichment analysis revealed that the eNOS activation pathway is deregulated only in SHR. Our study demonstrates that hypertension causes early proteomic changes in the renal vessels of SHR. These changes are relevant to vascular tone regulation and consequently may be involved in the development of vascular damage and hypertensive nephrosclerosis. Therefore, the identified proteins could be considered as therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-03-03_02:27:47.631.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jerome Zoidakis
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-29 13:44:56	ID requested
⏵ 1	2022-03-03 02:27:48	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Laser Capture Microdissection, vascular proteomics, essential hypertension, endothelial dysfunction, spontaneously hypertensive rats

Jerome Zoidakis
contact affiliation	Biotechnology Department, Proteomics Laboratory, BRFAA, Athens, Greece
contact email	izoidakis@bioacademy.gr
lab head
Jerome Zoidakis
contact affiliation	BRFAA
contact email	izoidakis@bioacademy.gr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008792
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis in microdissected renal vessels of hypertensive and normotensive rats