PXD008788

PXD008788 is an original dataset announced via ProteomeXchange.

Title	A Novel QconCAT-Based Proteomics Method for Determining Allele-Specific Protein Expression (ASPE)
Description	In the present study, we developed a novel targeted proteomics approach for quantification of allele-specific protein expression (ASPE) based on scheduled high resolution multiple reaction monitoring (sMRM-HR) with a heavy stable isotope-labeled quantitative concatamer (QconCAT) internal protein standard. This strategy was applied to the determination of the ASPE of UGT2B15 in human livers using the common UGT2B15 nonsynonymous variant rs1902023 (i.e. Y85D) as the marker to differentiate expressions from the two alleles. This novel ASPE approach has the potential to be widely utilized for the identification of cis-genetic variants capable of regulating gene expression at the protein level.
HostingRepository	PRIDE
AnnounceDate	2018-08-28
AnnouncementXML	Submission_2018-08-28_07:46:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian Shi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-29 03:46:14	ID requested
⏵ 1	2018-08-28 07:46:34	announced

Shi J, Wang X, Zhu H, Jiang H, Wang D, Nesvizhskii A, Zhu HJ, Determining Allele-Specific Protein Expression (ASPE) Using a Novel Quantitative Concatamer Based Proteomics Method. J Proteome Res, 17(10):3606-3612(2018) [pubmed]

submitter keyword: Human, Liver, Allele-Specific Protein Expression

Hao-Jie Zhu, Ph.D.
contact affiliation	Department of Clinical Pharmacy, University of Michigan College of Pharmacy
contact email	hjzhu@med.umich.edu
lab head
Jian Shi
contact affiliation	University of Michigan
contact email	jianshi@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD008788

PRIDE project URI

• PRIDE
  1. PXD008788
     1. Label: PRIDE project
     2. Name: A Novel QconCAT-Based Proteomics Method for Determining Allele-Specific Protein Expression (ASPE)