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PXD008786

PXD008786 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic study of Anton Chekhov' letters
DescriptionFive different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in A. Chekhov’s museum in Melikhovo (nearby Moscow), have been analyzed by applying to these surfaces EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C8 resins) diskettes. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectrometry. The environmental microbiota present on samples and the M. ycobacterium Tuberculosis tuberculosis strain were described by a meta-proteomics approach. Eight identified M. Tuberculosis tuberculosis proteins confirmed the presence of the bacterium and the cause of Chekhov death, in addition to several sequenced peptides belonging to other bacterial species. The human plasma proteins and human keratins, detected on a tiny blood spot on the shirt, demonstrated the power of the combined approach.
HostingRepositoryPRIDE
AnnounceDate2018-07-05
AnnouncementXMLSubmission_2018-07-05_02:49:08.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD008786
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlfonsina D'Amato
SpeciesList scientific name: Mycobacterium tuberculosis K; NCBI TaxID: 1249615;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-29 03:27:20ID requested
12018-07-05 02:49:10announced
Publication List
D'Amato A, Zilberstein G, Zilberstein S, Golovan MI, Zhuravleva AA, Righetti PG, Anton Chekhov and Robert Koch Cheek to Cheek: A Proteomic Study. Proteomics, 18(9):e1700447(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: EVA film
Mycobacterium tuberculosis
Cultural Heritage.
Contact List
Alfonsina D'Amato
contact affiliationPharmaceutical and Biopharmaceutical Analysis Unit Department of Pharmaceutical Sciences Università degli Studi di Milano Via L. Mangiagalli 25, 20133, Milano, Italia First floor, room 1066
contact emailalfonsina.damato@unimi.it
lab head
Alfonsina D'Amato
contact affiliationUniversità di Milano
contact emailalfonsina.damato@unimi.it
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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