PXD008777

PXD008777 is an original dataset announced via ProteomeXchange.

Title	Investigation of the effects on the proteome composition of Escherichia coli producing a recombinant protein in the periplasm under non-optimized and optimized conditions
Description	Recombinant proteins containing disulfide bonds, like antibody fragments, are usually produced in the periplasm of E. coli, because in this compartment of the E. coli cell the formation of disulfide bonds is catalyzed. A recombinant protein is targeted to the periplasm with the help of an N-terminally fused signal sequence, which is clipped off from the recombinant protein upon translocation across the cytoplasmic membrane. The single-chain variable antibody fragment BL1 N-terminally fused to the DsbA signal sequence was produced in the E. coli Lemo21(DE3) recombinant protein production strain at conditions non-optimal (0 µM L-rhamnose) and optimal (500 µM L-rhamnose) for the production of the scFv BL1 in the periplasm. Lemo21(DE3) cells not producing a recombinant protein were used as a reference.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_10:25:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jimmy Ytterberg
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-29 01:09:24	ID requested
⏵ 1	2019-11-12 10:25:34	announced

Baumgarten T, Ytterberg AJ, Zubarev RA, de Gier JW, Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress. Appl Environ Microbiol, 84(12):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Escherichia coli, proteomics, recombinant protein, protein production, periplasm, Lemo21(DE3)

Roman A. Zubarev
contact affiliation	Dept. Medical Biochemistry and Biophysics, Div. Chemistry I, Karolinska Institutet, Stockholm, Sweden
contact email	Roman.Zubarev@ki.se
lab head
Jimmy Ytterberg
contact affiliation	Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden
contact email	jimmy.ytterberg@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD008777

PRIDE project URI

• PRIDE
  1. PXD008777
     1. Label: PRIDE project
     2. Name: Investigation of the effects on the proteome composition of Escherichia coli producing a recombinant protein in the periplasm under non-optimized and optimized conditions