PXD008763

PXD008763 is an original dataset announced via ProteomeXchange.

Title	The unusual properties of the photosynthetic cytochrome c550 from the red algal lineage evidence additional biological roles
Description	Cytochrome c550 is an extrinsic component in the luminal side of photosystem II in cyanobacteria, as well as in eukaryotic algae from the red photosynthetic lineage including, among others, diatoms. By using strong and specific protease inhibitors we have confirmed that cytochrome c550 from the diatom Phaeodactylum tricornutum is expressed as a truncated form lacking the last hydrophobic residues at the C-terminal.. Phaeodactylum tricornutum cytochrome c550 seems to be modified at a post-translational level as the gene is appropriately transcribed. In addition, co-immunoprecipitation and mass spectrometry experiments have allowed the identification of novel interactions of Phaeodactylum tricornutum cytochrome c550 with proteins related to the CO2 concentrating mechanism and assimilation located in the chloroplast pyrenoid. Furthermore, immunoelectron microscopy analysis indicates that although cytochrome c550 is mainly located in the thylakoid domain of the chloroplast, it can also be found in the pyrenoid. The results are interpreted in terms of a specific protein modification associated to a higher PSII turnover or to new alternative functions of the heme protein, maybe related to carbon fixation.
HostingRepository	PRIDE
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-03-03_02:44:12.574.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jose Antonio Navarro
SpeciesList	scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-26 06:36:54	ID requested
⏵ 1	2022-03-03 02:44:12	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: ß-DM, ß-dodecyl-maltoside

CA, carbonic anhydrase

Cc550, cytochrome c550

Cc6, cytochrome c6

CCM, CO2-concentrating mechanism

DSS, disuccinimidyl suberate

FBA, fructose-bisphosphate aldolase

LC-MS/MS, liquid chromatography tandem-mass spectrometry

MALDI-TOF MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

MS, mass spectrometry

PSI, photosystem I

PSII, photosystem II

RubisCO, ribulose-1,5-bisphosphate carboxylase/oxygenase

Jose Antonio Navarro Carruesco
contact affiliation	CSIC-US, IBVF
contact email	jnavarro@ibvf.csic.es
lab head
Jose Antonio Navarro
contact affiliation	CSIC-US
contact email	jnavarro@ibvf.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD008763

PRIDE project URI

• PRIDE
  1. PXD008763
     1. Label: PRIDE project
     2. Name: The unusual properties of the photosynthetic cytochrome c550 from the red algal lineage evidence additional biological roles