PXD008748

PXD008748 is an original dataset announced via ProteomeXchange.

Title	Nucleolar-nucleoplasmic shuttling of the RNA-binding macrodomain-containing TARG1 is controlled by DNA damage-induced poly-ADP-ribose and implies functions in ribosome biogenesis
Description	In this project we employed affinity purification mass spectrometry to determine the interactome of the macrodomain containing protein TARG1/OARD1. TARG1 functions as a hydrolase towards mono-ADP-ribosylation (MARylation). TARG1 sowed strong binding to ribosomes and proteins associated with rRNA processing. However, when poly-ADP- ribosylation (PARylation) was present in the cellular lysate, TARG1 was found to interacts with DNA repair and chromatin-associated proteins, including ARTD1, in a PARylation-dependent manner. PARylation events also lead to a change in the subcellular localization of TARG1, which was found to reside in the nucleolus in resting cells, and shuttle to the nucleoplasm upon PARylation
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:13.471.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Preisinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-24 02:54:53	ID requested
1	2018-04-23 02:31:18	announced
2	2018-05-02 23:57:52	announced	Updated publication reference for PubMed record(s): 29712969.
⏵ 3	2024-10-22 04:04:14	announced	2024-10-22: Updated project metadata.

B, ü, tepage M, Preisinger C, von Kriegsheim A, Scheufen A, Lausberg E, Li J, Kappes F, Feederle R, Ernst S, Eckei L, Krieg S, M, ü, ller-Newen G, Rossetti G, Feijs KLH, Verheugd P, L, ü, scher B, Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription. Sci Rep, 8(1):6748(2018) [pubmed]

10.1038/s41598-018-25137-w;

curator keyword: Biomedical

submitter keyword: Human, AP-MS, ADP-ribosylation

Dr Christian Preisinger
contact affiliation	Proteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact email	cpreisinger@ukaachen.de
lab head
Christian Preisinger
contact affiliation	Proteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact email	cpreisinger@ukaachen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008748

PRIDE project URI

• PRIDE
  1. PXD008748
     1. Label: PRIDE project
     2. Name: Nucleolar-nucleoplasmic shuttling of the RNA-binding macrodomain-containing TARG1 is controlled by DNA damage-induced poly-ADP-ribose and implies functions in ribosome biogenesis