PXD008747

PXD008747 is an original dataset announced via ProteomeXchange.

Title	Histone variant H2A.Z deposition and acetylation directs the canonical Notch signaling response
Description	The study deals with the elucidation of potential interaction partners of the intracellular domain of the transmembrane receptor Notch1, termed NICD. The NICD is released from the cytoplasmic tail of the Notch receptor by gamma-secretase treatment and translocated as a transcription factor to the nucleus. Here, virally transduced recombinant NICD constructs (wild-type and deltaEP hyperactive mutant) were employed for AP-MS with the aim of identifying novel NICD interactors in a murine T cell leukemia cell line.
HostingRepository	PRIDE
AnnounceDate	2018-06-25
AnnouncementXML	Submission_2018-07-16_05:22:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Mittler
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-24 02:47:37	ID requested
1	2018-06-24 23:55:28	announced
2	2018-06-25 00:47:17	announced	Updated project metadata.
⏵ 3	2018-07-16 05:22:04	announced	Updated project metadata.

Giaimo BD, Ferrante F, Vallejo DM, Hein K, Gutierrez-Perez I, Nist A, Stiewe T, Mittler G, Herold S, Zimmermann T, Bartkuhn M, Schwarz P, Oswald F, Dominguez M, Borggrefe T, Histone variant H2A.Z deposition and acetylation directs the canonical Notch signaling response. Nucleic Acids Res, 46(16):8197-8215(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Orbitrap MS, AP-MS, MaxQuant, Notch, H2A.Z, histone variant, p400, Tip60, CRISPR

Gerhard Mittler
contact affiliation	Dr. Gerhard Mittler Proteomics Research Facility/Unit Max Planck Institute of Immunobiology and Epigenetics Stuebeweg 51, D-79108 Freiburg, Germany
contact email	mittler@ie-freiburg.mpg.de
lab head
Gerhard Mittler
contact affiliation	Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics
contact email	mittler@ie-freiburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008747

PRIDE project URI

• PRIDE
  1. PXD008747
     1. Label: PRIDE project
     2. Name: Histone variant H2A.Z deposition and acetylation directs the canonical Notch signaling response