PXD008746

PXD008746 is an original dataset announced via ProteomeXchange.

Title	Xpo7 is a broad-spectrum exportin and a nuclear import receptor
Description	Cargo molecules which exceed the passive diffusion limit of ~5nm require active translocation to cross the nuclear pore complex (NPC). Such a facilitated transport is achieved by specialized nuclear transport receptors (NTRs), which are classified according to their shuttling direction. Exportins bind their cargo in a RanGTP-dependent manner inside the nucleus and release it in the cytoplasm. Importins, on the other hand, bind their cargo without RanGTP and transfer it in the opposite direction. CRM1/Xpo1 is one of the best-characterized NTRs mostly due to the availability of specific inhibitors, like leptomycin B, which allowed cargo validation in vivo. Such inhibitors have not been characterized for other NTRs, thus analysis of those lagged behind. Here, we developed nanobodies to specifically target exportin 7/Xpo7 and block its transport pathway. Moreover, in-depth MS/MS analysis of Xpo7 under nuclear (+RanGTP) and cytoplasmic conditions (-RanGTP) revealed novel binders including ~200 potential export substrates, but also ~30 nuclear import cargoes. Enrichment factors of a putative cargo were calculated using the iBAQ strategy to quantify detectable proteins in the input as well as in the import or export mimicking material. Validation of selected cargo molecules was then accomplished by anti-Xpo7 nanobodies causing cargo mislocalization when transfected into cultured cells. Collectively, the data establish Xpo7 as a bidirectional NTR with a broad substrate specificity.
HostingRepository	PRIDE
AnnounceDate	2018-05-07
AnnouncementXML	Submission_2018-05-23_00:37:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christin Kappert
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-24 02:41:00	ID requested
1	2018-05-07 00:26:56	announced
⏵ 2	2018-05-23 00:37:17	announced	Updated publication reference for PubMed record(s): 29748336.

Aksu M, Pleiner T, Karaca S, Kappert C, Dehne HJ, Seibel K, Urlaub H, Bohnsack MT, G, ö, rlich D, Xpo7 is a broad-spectrum exportin and a nuclear import receptor. J Cell Biol, 217(7):2329-2340(2018) [pubmed]

curator keyword: Biological

submitter keyword: Exportin, Importin, NPCs, Nanobodies, RanGTPase, Erythropoiesis, LC-MSMS, mouse

Prof. Dirk Görlich
contact affiliation	Department of cellular Logistics
contact email	goerlich@mpibpc.mpg.de
lab head
Christin Kappert
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christin.kappert@mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008746
     1. Label: PRIDE project
     2. Name: Xpo7 is a broad-spectrum exportin and a nuclear import receptor