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PXD008745

PXD008745 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleN-glycoproteomic analyses from different insect species reveal conserved and unique glycoproteins
DescriptionN-glycosylation is one of the most abundant and conserved protein modifications in eukaryotes. This modification serves various important functions, such as protein folding and cellular attachment, but also modulation of a protein’s function. Recently, it has been shown that N-glycosylation of proteins plays a vital role in insect development and survival, which makes it an interesting target for pest control. Despite the importance of protein N-glycosylation in insects, not much is known about insect N-glycoproteomes. Here, we report on the N-glycoproteomes of three major pest insects spanning different insect orders; Drosophila melanogaster (Diptera), Tribolium castaneum (Coleoptera) and Acyrthosiphon pisum (Hemiptera). The number of identified N-glycosylation sites ranged from 889 in T. castaneum, to 941 in D. melanogaster and 1,338 in A. pisum. Comparison between the different insect species revealed both conserved and species-specific glycoproteins. The functionality of the insect glycoproteins together with the conservation of the N-glycosites throughout evolution are discussed. This information can help in the elaboration of novel pest insect control strategies based on interference in insect glycosylation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:04:13.096.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAn Staes
SpeciesList scientific name: Tetropium castaneum; NCBI TaxID: 256648; scientific name: Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium); NCBI TaxID: 118099; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListdeamidated residue
InstrumentQ Exactive HF; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-24 02:30:45ID requested
12018-06-18 01:15:07announced
22024-10-22 04:04:15announced2024-10-22: Updated project metadata.
Publication List
Scheys F, Van Damme EJM, De Schutter K, Staes A, Gevaert K, Smagghe G, Evolutionarily conserved and species-specific glycoproteins in the N-glycoproteomes of diverse insect species. Insect Biochem Mol Biol, 100():22-29(2018) [pubmed]
10.1016/j.ibmb.2018.04.011;
Keyword List
curator keyword: Biological
submitter keyword: glycoprotein, mass spectrometry (MS), proteomics,Drosophila melanogaster, insect, pest insect, lectin, N-linked glycosylation
Contact List
Guy Smagghe
contact affiliationDepartment of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent,Belgium
contact emailguy.smagghe@ugent.be
lab head
An Staes
contact affiliationMedical Protein Chemistry
contact emailan.staes@vib-ugent.be
dataset submitter
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Dataset FTP location
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PRIDE project URI
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