PXD008726

PXD008726 is an original dataset announced via ProteomeXchange.

Title	Mouse WT vs. A20 KO BMDM differential proteomics
Description	A20 is a negative regulator of NF-κB signaling, crucial to control inflammatory responses and ensure tissue homeostasis. A20 is thought to restrict NF-κB activation both by its ubiquitin-editing activity as by non-enzymatic activities. Besides its role in NF-κB signaling, A20 also acts as a protective factor inhibiting apoptosis and necroptosis. Because of the ability of A20 to both ubiquitinate and deubiquitinate substrates and its involvement in many cellular processes, we hypothesized that deletion of A20 might generally impact on protein levels, thereby disrupting cellular processes. We performed a differential proteomics study of bone marrow derived macrophages (BMDMs) from control and myeloid-specific A20 knockout mice, both in untreated conditions and after LPS and TNF treatment, and demonstrate proteome-wide changes in protein expression upon A20 deletion. Several inflammatory proteins are up-regulated in the absence of A20, even without an inflammatory stimulus. Depending on the treatment and the time, more proteins are regulated. Together these changes may affect multiple signaling pathways disturbing tissue homeostasis and inducing (autoimmune) inflammation, as suggested by genetic studies in patients.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:05.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmy Van Quickelberghe
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-19 07:36:21	ID requested
1	2018-05-15 03:57:45	announced
⏵ 2	2024-10-22 04:04:08	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.8b00139;

Van Quickelberghe E, Martens A, Goeminne LJE, Clement L, van Loo G, Gevaert K, Identification of Immune-Responsive Gene 1 (IRG1) as a Target of A20. J Proteome Res, 17(6):2182-2191(2018) [pubmed]

curator keyword: Biological

submitter keyword: A20, LC-MSMS,BMDM

Kris Gevaert
contact affiliation	VIB-UGent Center for Medical Biotechnology Department of Biochemistry, Ghent University Ghent, Belgium
contact email	kris.gevaert@vib-ugent.be
lab head
Emmy Van Quickelberghe
contact affiliation	Ghent University
contact email	emmy.vanquickelberghe@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD008726

PRIDE project URI

• PRIDE
  1. PXD008726
     1. Label: PRIDE project
     2. Name: Mouse WT vs. A20 KO BMDM differential proteomics