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PXD008721 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNannochloropsis oceanica cultivations display phenotypic heterogeneity in lipid metabolism
DescriptionBy combination of FACS sorting based on lipid sensitive Nile Red staining and small cell number proteomics the occurrence of population heterogeneity was analyzed. First population heterogeneity was identified by Nile Red staining and FACS in N. oceanica cells grown in nitrogen replete and nitrogen deplete media. For each condition two subpopulations(+NP3,+NP4,-NP3,-NP4) were detected and three replicates were sorted. Using the gathered proteome data proteins were identified and quantified with the MaxQuant software. Based on these quantification results a two sample t-test was performed to show significant regulation between +NP3 vs. +NP4 or -NP3 vs. -NP4. Goal was to understand the biological reasons for differences in lipid production between subpopulations.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Harst
SpeciesList scientific name: Nannochloropsis oceanica strain IMET1; NCBI TaxID: 1333499;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-19 03:56:31ID requested
12019-02-15 04:16:49announced
Publication List
Chen C, Harst A, You W, Xu J, Ning K, Poetsch A, . Biotechnol Biofuels, 12():21(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Population heterogeneity
Nannochloropsis oceanica
lipid metabolism
small cell number proteomics
Contact List
Dr. Ansgar Poetsch
contact affiliationSchool of Biomedical and Healthcare Sciences, Plymouth University, Plymouth PL4 8AA, United Kingdom
contact emailansgar.poetsch@plymouth.ac.uk
lab head
Andreas Harst
contact affiliationRuhr University Bochum
contact emailandreas.harst@ruhr-uni-bochum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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