PXD008707 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Encoding the human serine phosphoproteome in an engineered bacterial system |
Description | We synthesize a synthetic representation of the human serine phosphoproteome, expressed heterologously as >=31 amino acid phosphopeptides in an engineered strain of E. coli. We confirm that tens of thousands of these phosphopeptides can be successfully expressed and retain important functional characteristics of serine phosphorylation as it occurs in native eukaryotic systems. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:04:02.396.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Karl Barber |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-01-18 01:06:58 | ID requested | |
1 | 2018-04-17 01:03:24 | announced | |
2 | 2018-06-13 01:56:05 | announced | Updated publication reference for PubMed record(s): 29889213. |
⏵ 3 | 2024-10-22 04:04:07 | announced | 2024-10-22: Updated project metadata. |
Publication List
Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J, Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Nat Biotechnol, 36(7):638-644(2018) [pubmed] |
10.1038/nbt.4150; |
Keyword List
submitter keyword: phosphorylation, phosphoproteomics,LC-MSMS |
Contact List
Jesse Rinehart |
contact affiliation | Yale University Department of Cellular & Molecular Physiology Systems Biology Institute USA |
contact email | jesse.rinehart@yale.edu |
lab head | |
Karl Barber |
contact affiliation | Yale University |
contact email | karl.barber@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008707 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008707
- Label: PRIDE project
- Name: Encoding the human serine phosphoproteome in an engineered bacterial system