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PXD008707

PXD008707 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEncoding the human serine phosphoproteome in an engineered bacterial system
DescriptionWe synthesize a synthetic representation of the human serine phosphoproteome, expressed heterologously as >=31 amino acid phosphopeptides in an engineered strain of E. coli. We confirm that tens of thousands of these phosphopeptides can be successfully expressed and retain important functional characteristics of serine phosphorylation as it occurs in native eukaryotic systems.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:04:02.396.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarl Barber
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-18 01:06:58ID requested
12018-04-17 01:03:24announced
22018-06-13 01:56:05announcedUpdated publication reference for PubMed record(s): 29889213.
32024-10-22 04:04:07announced2024-10-22: Updated project metadata.
Publication List
Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J, Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Nat Biotechnol, 36(7):638-644(2018) [pubmed]
10.1038/nbt.4150;
Keyword List
submitter keyword: phosphorylation, phosphoproteomics,LC-MSMS
Contact List
Jesse Rinehart
contact affiliationYale University Department of Cellular & Molecular Physiology Systems Biology Institute USA
contact emailjesse.rinehart@yale.edu
lab head
Karl Barber
contact affiliationYale University
contact emailkarl.barber@yale.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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