PXD008707

PXD008707 is an original dataset announced via ProteomeXchange.

Title	Encoding the human serine phosphoproteome in an engineered bacterial system
Description	We synthesize a synthetic representation of the human serine phosphoproteome, expressed heterologously as >=31 amino acid phosphopeptides in an engineered strain of E. coli. We confirm that tens of thousands of these phosphopeptides can be successfully expressed and retain important functional characteristics of serine phosphorylation as it occurs in native eukaryotic systems.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:02.396.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karl Barber
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-18 01:06:58	ID requested
1	2018-04-17 01:03:24	announced
2	2018-06-13 01:56:05	announced	Updated publication reference for PubMed record(s): 29889213.
⏵ 3	2024-10-22 04:04:07	announced	2024-10-22: Updated project metadata.

Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J, Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Nat Biotechnol, 36(7):638-644(2018) [pubmed]

10.1038/nbt.4150;

submitter keyword: phosphorylation, phosphoproteomics,LC-MSMS

Jesse Rinehart
contact affiliation	Yale University Department of Cellular & Molecular Physiology Systems Biology Institute USA
contact email	jesse.rinehart@yale.edu
lab head
Karl Barber
contact affiliation	Yale University
contact email	karl.barber@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008707

PRIDE project URI

• PRIDE
  1. PXD008707
     1. Label: PRIDE project
     2. Name: Encoding the human serine phosphoproteome in an engineered bacterial system