PXD008706 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Systematic Protein Turnover Map for Decoding Protein Degradation |
Description | Protein degradation is mediated by an expansive and complex network of protein modification and degradation enzymes. Matching degradation enzymes with their targets and determining globally which proteins are degraded by the proteasome or lysosome/vacuole have been a major challenge. Furthermore, an integrated view of protein degradation for cellular pathways has been lacking. Here, we present an analytical platform that combines systematic gene deletions with quantitative measures of protein turnover to deconvolve protein degradation pathways for Saccharomyces cerevisiae . The resulting turnover map (T-MAP) reveals target candidates of nearly all E2 and E3 ubiquitin ligases and identifies the primary degradation routes for most proteins. We further mined this T-MAP to identify new substrates of ER-associated degradation (ERAD) involved in sterol biosynthesis and to uncover regulatory nodes for sphingolipid biosynthesis. The T-MAP approach should be broadly applicable to the study of other cellular processes, including mammalian systems. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:17:30.498.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | romain christiano |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-01-18 00:28:53 | ID requested | |
1 | 2021-01-05 02:16:48 | announced | |
⏵ 2 | 2024-10-22 05:17:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
Christiano R, Arlt H, Kabatnik S, Mejhert N, Lai ZW, Farese RV, Walther TC, A Systematic Protein Turnover Map for Decoding Protein Degradation. Cell Rep, 33(6):108378(2020) [pubmed] |
10.1016/j.celrep.2020.108378; |
Keyword List
curator keyword: Biological |
submitter keyword: profiling, turnover, pulse SILAC,yeast |
Contact List
Romain Christiano |
contact affiliation | Genetics and Complex Diseases Harvard School of Public Health 677 Huntington Ave Boston, MA 02115 |
contact email | rchrist@hsph.harvar.edu |
lab head | |
romain christiano |
contact affiliation | Harvard School of Public Health |
contact email | rchrist@hsph.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008706
- Label: PRIDE project
- Name: A Systematic Protein Turnover Map for Decoding Protein Degradation