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PXD008706

PXD008706 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Systematic Protein Turnover Map for Decoding Protein Degradation
DescriptionProtein degradation is mediated by an expansive and complex network of protein modification and degradation enzymes. Matching degradation enzymes with their targets and determining globally which proteins are degraded by the proteasome or lysosome/vacuole have been a major challenge. Furthermore, an integrated view of protein degradation for cellular pathways has been lacking. Here, we present an analytical platform that combines systematic gene deletions with quantitative measures of protein turnover to deconvolve protein degradation pathways for Saccharomyces cerevisiae . The resulting turnover map (T-MAP) reveals target candidates of nearly all E2 and E3 ubiquitin ligases and identifies the primary degradation routes for most proteins. We further mined this T-MAP to identify new substrates of ER-associated degradation (ERAD) involved in sterol biosynthesis and to uncover regulatory nodes for sphingolipid biosynthesis. The T-MAP approach should be broadly applicable to the study of other cellular processes, including mammalian systems.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:17:30.498.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterromain christiano
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-18 00:28:53ID requested
12021-01-05 02:16:48announced
22024-10-22 05:17:33announced2024-10-22: Updated project metadata.
Publication List
Christiano R, Arlt H, Kabatnik S, Mejhert N, Lai ZW, Farese RV, Walther TC, A Systematic Protein Turnover Map for Decoding Protein Degradation. Cell Rep, 33(6):108378(2020) [pubmed]
10.1016/j.celrep.2020.108378;
Keyword List
curator keyword: Biological
submitter keyword: profiling, turnover, pulse SILAC,yeast
Contact List
Romain Christiano
contact affiliationGenetics and Complex Diseases Harvard School of Public Health 677 Huntington Ave Boston, MA 02115
contact emailrchrist@hsph.harvar.edu
lab head
romain christiano
contact affiliationHarvard School of Public Health
contact emailrchrist@hsph.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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