PXD008706

PXD008706 is an original dataset announced via ProteomeXchange.

Title	A Systematic Protein Turnover Map for Decoding Protein Degradation
Description	Protein degradation is mediated by an expansive and complex network of protein modification and degradation enzymes. Matching degradation enzymes with their targets and determining globally which proteins are degraded by the proteasome or lysosome/vacuole have been a major challenge. Furthermore, an integrated view of protein degradation for cellular pathways has been lacking. Here, we present an analytical platform that combines systematic gene deletions with quantitative measures of protein turnover to deconvolve protein degradation pathways for Saccharomyces cerevisiae . The resulting turnover map (T-MAP) reveals target candidates of nearly all E2 and E3 ubiquitin ligases and identifies the primary degradation routes for most proteins. We further mined this T-MAP to identify new substrates of ER-associated degradation (ERAD) involved in sterol biosynthesis and to uncover regulatory nodes for sphingolipid biosynthesis. The T-MAP approach should be broadly applicable to the study of other cellular processes, including mammalian systems.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:30.498.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	romain christiano
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-18 00:28:53	ID requested
1	2021-01-05 02:16:48	announced
⏵ 2	2024-10-22 05:17:33	announced	2024-10-22: Updated project metadata.

Christiano R, Arlt H, Kabatnik S, Mejhert N, Lai ZW, Farese RV, Walther TC, A Systematic Protein Turnover Map for Decoding Protein Degradation. Cell Rep, 33(6):108378(2020) [pubmed]

10.1016/j.celrep.2020.108378;

curator keyword: Biological

submitter keyword: profiling, turnover, pulse SILAC,yeast

Romain Christiano
contact affiliation	Genetics and Complex Diseases Harvard School of Public Health 677 Huntington Ave Boston, MA 02115
contact email	rchrist@hsph.harvar.edu
lab head
romain christiano
contact affiliation	Harvard School of Public Health
contact email	rchrist@hsph.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD008706

PRIDE project URI

• PRIDE
  1. PXD008706
     1. Label: PRIDE project
     2. Name: A Systematic Protein Turnover Map for Decoding Protein Degradation