PXD008704

PXD008704 is an original dataset announced via ProteomeXchange.

Title	Uncoupled activation and cyclisation in catmint reductive terpenoid biosynthesis
Description	Terpenes synthases typically form complex molecular scaffolds by concerted activation and cyclization of linear starting materials in a single active site. We have determined that iridoid synthase, an atypical reductive terpene synthase, catalyses the activation of its substrate 8-oxogeranial into a reactive enol intermediate but does not catalyse the subsequent cyclisation into nepetalactol. This discovery led us to identify a class of nepetalactol-related short-chain dehydrogenase enzymes (NEPS) from catmint (Nepeta mussinii) which catalyse the stereoselective cyclisation of the enol intermediate into nepetalactol isomers. Subsequent oxidation of nepetalactols by NEPS1 provides nepetalactones, metabolites that are well known for both insect-repellent activity and euphoric effect in cats. Structural characterisation of the NEPS3 cyclase reveals it binds to NAD+ yet does not utilise it chemically for a non-oxidoreductive formal [4+2] cyclisation. These discoveries will complement metabolic reconstructions of iridoid and monoterpene indole alkaloid biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2019-08-12
AnnouncementXML	Submission_2019-08-12_01:50:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Nepeta mussinii; NCBI TaxID: 213401;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-17 07:37:51	ID requested
⏵ 1	2019-08-12 01:50:07	announced

Lichman BR, Kamileen MO, Titchiner GR, Saalbach G, Stevenson CEM, Lawson DM, O'Connor SE, Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis. Nat Chem Biol, 15(1):71-79(2019) [pubmed]

curator keyword: Biological

submitter keyword: iridoid, catmint, nepetalactone, trichome, proteomics, LC-MSMS

Sarah O'Connor
contact affiliation	Department of Biological Chemistry John Innes Centre Norwich Research Park Norwich NR4 7UH
contact email	Sarah.OConnor@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008704
     1. Label: PRIDE project
     2. Name: Uncoupled activation and cyclisation in catmint reductive terpenoid biosynthesis