PXD008694

PXD008694 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Role of the Visual Conditioning-Dependent Nascent Proteome in Neuronal Plasticity
Description	Experience-dependent synaptic plasticity refines brain circuits during development. To uncover protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we performed quantitative proteomic analysis of the nascent proteome using improved bio-orthogonal metabolic labeling (BONCAT) to identify candidate plasticity proteins (CPPs) that undergo differential protein synthesis in response to visual conditioning (VC) in Xenopus optic tectum. We identified 83 CPPs that formed strongly connected networks and were annotated to a variety of biological functions, including RNA splicing, protein translation, and chromatin remodeling. Functional analysis of select CPPs using translation blocking morpholinos revealed the requirement of eukaryotic initiation factor 3 subunit A (eIF3A), fused in sarcoma (FUS), and ribosomal protein s17 (RPS17) in experience-dependent structural plasticity of tectal neurons. These results demonstrate that the nascent proteome is dynamic in response to VC and that de novo synthesis of the machinery that regulates gene expression and protein translation is required for experience-dependent structural plasticity.
HostingRepository	MassIVE
AnnounceDate	2018-02-07
AnnouncementXML	Submission_2018-02-07_10:07:32.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel McClatchy
SpeciesList	scientific name: Xenopus laevis/gilli; NCBI TaxID: 8359;
ModificationList	AHA-Alkyne; Dimethyl; Dimethyl:2H(4)
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-16 11:27:47	ID requested
⏵ 1	2018-02-07 10:07:32	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Azidohomoalanine, plasticity, neurons

submitter keyword: Azidohomoalanine, plasticity, neurons

Contact List

Hollis T. Cline
contact affiliation	The Scripps Research Institute
contact email	cline@scripps.edu
lab head
Daniel McClatchy
contact affiliation	TSRI
contact email	dmcclat@scripps.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081728