PXD008686

PXD008686 is an original dataset announced via ProteomeXchange.

Title	The MYO6 interactome using BioID
Description	The intracellular function of myosin motors requires a number of adaptor molecules, which control cargo attachment, but also fine-tune motor activity in time and space. These motor-adaptor-cargo interactions are often weak, transient or highly regulated. To overcome these problems we use a proximity labelling-based proteomics strategy (BioID) to map the interactome of the unique minus end-directed actin motor MYO6. Our analysis identified several distinct MYO6-adaptor modules including two complexes containing RHO GEFs which we screened using further BioID baits (LRCH3, DOCK7, GIPC1 and ARHGEF12). These complexes emphasise the multifunctionality of MYO6 provides the first in vivo interactome of a myosin motor protein, highlighting the power of this approach in uncovering dynamic and functionally diverse myosin motor complexes.
HostingRepository	PRIDE
AnnounceDate	2018-02-27
AnnouncementXML	Submission_2018-02-27_04:49:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tom O'Loughlin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-16 05:02:03	ID requested
⏵ 1	2018-02-27 04:49:43	announced

O'Loughlin T, Masters TA, Buss F, The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics. EMBO Rep, 19(4):(2018) [pubmed]

curator keyword: Biological

submitter keyword: MYO6, BioID

Dr. Folma Buss
contact affiliation	Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, UK
contact email	fb207@cam.ac.uk
lab head
Tom O'Loughlin
contact affiliation	CIMR
contact email	to277@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008686

PRIDE project URI

• PRIDE
  1. PXD008686
     1. Label: PRIDE project
     2. Name: The MYO6 interactome using BioID