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PXD008685

PXD008685 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis
DescriptionAggregation is a sequence-specific process, nucleated by short aggregation prone regions (APR) that can be exploited to induce aggregation of proteins containing the same APR. Here we find that most APRs are unique within a proteome, but that a small minority of APRs occur in many proteins. When aggregation is nucleated in bacteria by such frequently occurring APRs, it leads to massive and lethal inclusion body formation containing a large number of proteins. Build-up of bacterial resistance against these peptides is slow. In addition, the approach is effective against drug-resistant clinical isolates of E. coli and A. baumannii, reducing bacterial load in a murine bladder infection model. Our results indicate that redundant APRs are weak points of bacterial protein homeostasis and that targeting these may be an attractive antibacterial strategy.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:07:14.961.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD008685
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLadan Khodaparast
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListAmmonia-loss; Carboxymethyl; Dehydrated; Oxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-16 01:15:37ID requested
12018-03-05 01:57:23announced
22024-10-22 04:07:15announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD008685;
Khodaparast L, Khodaparast L, Gallardo R, Louros NN, Michiels E, Ramakrishnan R, Ramakers M, Claes F, Young L, Shahrooei M, Wilkinson H, Desager M, Mengistu Tadesse W, Nilsson KPR, Hammarstr, ö, m P, Aertsen A, Carpentier S, Van Eldere J, Rousseau F, Schymkowitz J, Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis. Nat Commun, 9(1):866(2018) [pubmed]
10.1038/s41467-018-03131-0;
Keyword List
ProteomeXchange project tag: Protein Misfolding and Aggregation (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP)
curator keyword: Biological
submitter keyword: Aggregation prone regions,Protein aggregation, Bacteria
Contact List
Joost Schymkowitz
contact affiliationSwitch Laboratory VIB‐KU Leuven Center for Brain & Disease Research Department of Cellular and Molecular Medicine
contact emailjoost.schymkowitz@kuleuven.vib.be
lab head
Ladan Khodaparast
contact affiliationVIB‐KU Leuven Center for Brain & Disease Research Department of Cellular and Molecular Medicine
contact emailLadan.khodaparast@kuleuven.vib.be
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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