<<< Full experiment listing

PXD008684

PXD008684 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn iTRAQ-based proteomics screen analysis of potential regulators of wheat (Triticum aestivum L.) root growth inhibition due to Al stress
DescriptionAluminum (Al)-induced root tip cell elongation inhibition is a major cause of plant root elongation suppression. Root tip cell elongation inhibition is caused by disruption to cell wall component metabolism, growth signaling, or cellular damage. The aim of this study was to identify the proteins involved in root elongation under Al stress in the tolerant wheat (Triticum aestivum L.) ET8. We investigated wheat root protein profiles using isobaric tags for relative and absolute quantification (iTRAQ). In total, 97 differentially expressed proteins from Al-tolerant wheat roots were screened and 11 of the 97 proteins were root elongation related. The 14-3-3 protein, plasma membrane ATPase, phospholipase D, and peroxidase are involved in cell wall component metabolism. The cellulase activity and callase activity were down-regulated by Al stress, whereas, the PAL, CAD, and POD activities were up-regulated. In addition, the callose, cellulose, lignin, and H2O2 contents varied significantly. Furthermore, Al stress inhibited root elongation in a metabolic enzyme and carbohydrate-dependent manner. The results indicated that the protein involved with growth signaling is elongation factor 1-α. The cellular damage related proteins are glycosyltransferase and sucrose 1-fructosyltransferase. The iTRAQ analysis highlighted candidate proteins associated with root cell elongation and revealed several new aspects of the metabolic processes underlying Al toxicity and tolerance. We propose that Al stress leads to marked variations in metabolic enzyme activity, carbohydrate content, and cellular damage, followed by inhibition of root elongation in wheat roots. This study has provided the most integrated view of the adaptive root responses to Al-toxicity.
HostingRepositoryiProX
AnnounceDate2018-01-15
AnnouncementXMLSubmission_2019-12-12_20:05:47.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYang
SpeciesList scientific name: Triticum aestivum; NCBI TaxID: 4565; scientific name: Triticum aestivum subsp. tibeticum; NCBI TaxID: 231718;
ModificationListiTRAQ8plex reporter+balance reagent acylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-15 21:11:35ID requested
12018-01-15 21:19:39announced
22018-01-15 22:09:41announcedUpdate keyword information.
32019-12-12 20:05:48announced2019-12-13: Update publication information.
Publication List
Yang Y, Ma L, Zeng H, Chen LY, Zheng Y, Li CX, Yang ZP, Wu N, Mu X, Dai CY, Guan HL, Cui XM, Liu Y, iTRAQ-based proteomics screen for potential regulators of wheat (Triticum aestivum L.) root cell wall component response to Al stress. Gene, 675():301-311(2018) [pubmed]
Keyword List
submitter keyword: Aluminum-toxicity
iTRAQ
proteomics
Triticum aestivum L
root growth
metabolism
Contact List
Ye Yang
contact affiliationKey Laboratory of Sustainable Development and Utilization of Famous-region Drug, Key Laboratory of Panax Notoginseng Resources Sustainable Development and Utilization of State Administration of Traditional Chinese Medicine
contact emailyangyekm@163.com
lab head
Yang
contact affiliationKunming University of Science and Technology
contact emaillyhj_2002@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI