PXD008676

PXD008676 is an original dataset announced via ProteomeXchange.

Title	Pseudouridylation of tRNA-derived fragments steers translational control in stem cells
Description	Pseudouridylation (Ψ) is the most abundant and widespread type of RNA epigenetic modification in living organisms; however, the biological role of Ψ remains poorly understood. Here, we show that a Ψ-driven posttranscriptional program steers translation control to impact stem cell commitment during early embryogenesis. Mechanistically, the Ψ ‘writer’ PUS7 modifies and activates a network of tRNA-derived fragments (tRFs) targeting the translation initiation complex. PUS7 inactivation in embryonic stem cells impairs tRF-mediated translational regulation leading to increased protein biosynthesis and abnormal germ layer specification. Remarkably, dysregulation of PUS7 and tRFs in myeloid malignancies associates with altered translation rates, suggesting a role of Ψ in leukemogenesis. Our findings unveil a critical function of Ψ in directing translational control in stem cells with important implications for human disease.
HostingRepository	PRIDE
AnnounceDate	2018-04-11
AnnouncementXML	Submission_2018-04-11_05:00:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristyna Pimkova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2x(13)C: 4x(2)H labeled dimethylated residue; 4x(2)H labeled dimethylated residue; N-acetylated residue; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-15 01:54:55	ID requested
⏵ 1	2018-04-11 05:00:43	announced

Guzzi N, Cie, ś, la M, Ngoc PCT, Lang S, Arora S, Dimitriou M, Pimkov, á K, Sommarin MNE, Munita R, Lubas M, Lim Y, Okuyama K, Soneji S, Karlsson G, Hansson J, J, ö, nsson G, Lund AH, Sigvardsson M, Hellstr, ö, m-Lindberg E, Hsieh AC, Bellodi C, Pseudouridylation of tRNA-Derived Fragments Steers Translational Control in Stem Cells. Cell, 173(5):1204-1216.e26(2018) [pubmed]

curator keyword: Biological

submitter keyword: pseudouridylation, tRNA, dimethyl labelling

Jenny Pyl Hansson
contact affiliation	Proteomic Hematology Division of Molecular Hematology Lund Stem Cell Center, Lund University BMC B12, 221 84 Lund, Sweden
contact email	jenny.hansson@med.lu.se
lab head
Kristyna Pimkova
contact affiliation	Lund University
contact email	kristyna.pimkova@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008676

PRIDE project URI

• PRIDE
  1. PXD008676
     1. Label: PRIDE project
     2. Name: Pseudouridylation of tRNA-derived fragments steers translational control in stem cells