PXD008673

PXD008673 is an original dataset announced via ProteomeXchange.

Title	Neisseria gonorrhoeae MlaA inversely influences gonococcal virulence and membrane vesicle production
Description	The six-component maintenance of lipid asymmetry (Mla) system is responsible for retrograde transport of phospholipids, ensuring the barrier function of the Gram-negative cell envelope. Located within the outer membrane MlaA (VacJ) likely acts as a channel to shuttle phospholipids from the outer leaflet, bypassing the inner leaflet. In Neisseria gonorrhoeae, we previously discovered MlaA, encoded by the ngo2121 genetic locus, as a proteome-derived new vaccine and therapeutic target against this serious public health threat. Our follow-up with phenotype microarrays linked the loss of MlaA with an extensive chemical sensitivity phenome. For the current study, we investigated the role of gonococcal MlaA in bacterial physiology and pathogenicity. Quantitative proteomics were applied to cell envelopes and membrane vesicles derived from wild type and ∆mlaA bacteria to examine the alterations in protein composition induced by the loss of MlaA from the outer membrane, as well as to determine whether the abundance of known or potential virulence factors was altered in the mutant. We determined that membrane vesicles were more highly affected by the loss of MlaA than were cell envelopes, and that both cell envelopes and membrane vesicles derived from the ∆mlaA mutant were enriched with adhesins and other virulence factors.
HostingRepository	PRIDE
AnnounceDate	2019-03-13
AnnouncementXML	Submission_2019-03-12_21:14:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Sikora
SpeciesList	scientific name: Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090); NCBI TaxID: 242231;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-15 01:39:57	ID requested
⏵ 1	2019-03-12 21:14:57	announced

Baarda BI, Zielke RA, Le Van A, Jerse AE, Sikora AE, Neisseria gonorrhoeae MlaA influences gonococcal virulence and membrane vesicle production. PLoS Pathog, 15(3):e1007385(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Neisseria gonorrhoeae, membrane vesicles, mlaA

Aleksandra E. Sikora
contact affiliation	Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon 97331, USA
contact email	aleksandra.sikora@oregonstate.edu
lab head
Aleksandra Sikora
contact affiliation	College of Pharmacy
contact email	aleksandra.sikora@oregonstate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD008673

PRIDE project URI

• PRIDE
  1. PXD008673
     1. Label: PRIDE project
     2. Name: Neisseria gonorrhoeae MlaA inversely influences gonococcal virulence and membrane vesicle production