PXD008659

PXD008659 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus
Description	Neural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used in vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with the methionine analog azidohomoalanine (AHA) combined with the multidimensional protein identification technique (MudPIT) to identify proteins that are synthesized in the tadpole brain over 24 hr. We induced conditioning-dependent plasticity of visual avoidance behavior, which required N-methyl-D-aspartate (NMDA) and Ca(2+)-permeable alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, alphaCaMKII, and rapid protein synthesis. Combining BONCAT with western blots revealed that proteins including alphaCaMKII, MEK1, CPEB, and GAD65 are synthesized during conditioning. Acute synthesis of CPEB during conditioning is required for behavioral plasticity as well as conditioning-induced synaptic and structural plasticity in the tectal circuit. We outline a signaling pathway that regulates protein-synthesis-dependent behavioral plasticity in intact animals, identify newly synthesized proteins induced by visual experience, and demonstrate a requirement for acute synthesis of CPEB in plasticity.
HostingRepository	MassIVE
AnnounceDate	2018-01-16
AnnouncementXML	Submission_2018-01-16_13:03:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel McClatchy
SpeciesList	scientific name: Xenopus laevis; common name: African clawed frog; NCBI TaxID: 8355;
ModificationList	AHA-Alkyne
Instrument	LTQ Orbitrap XL

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-11 14:15:31	ID requested
⏵ 1	2018-01-16 13:03:04	announced

Publication List

Shen W, Liu HH, Schiapparelli L, McClatchy D, He HY, Yates JR, Cline HT, Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus. Cell Rep, 6(4):737-47(2014) [pubmed]

Shen W, Liu HH, Schiapparelli L, McClatchy D, He HY, Yates JR, Cline HT, Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus. Cell Rep, 6(4):737-47(2014) [pubmed]

Keyword List

submitter keyword: Azidohomoalanine, MudPIT, Xenopus

submitter keyword: Azidohomoalanine, MudPIT, Xenopus

Contact List

Hollis T. Cline
contact affiliation	The Scripps Research Institute
contact email	cline@scripps.edu
lab head
Daniel McClatchy
contact affiliation	TSRI
contact email	dmcclat@scripps.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081914