PXD008656

PXD008656 is an original dataset announced via ProteomeXchange.

Title	Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics
Description	We investigated the compatibility of tube-gel with alternatives to SDS-based buffers allowing notably the extraction of proteins in various pH conditions. We also explored the use of photopolymerization to extend the number of possibilities, as it is compatible with a wide range of pH and is non-oxydative. To achieve this goal, we compared six extraction buffers in combination with two polymerization conditions to further optimize tube-gel protocol and evaluate its versatility.
HostingRepository	PRIDE
AnnounceDate	2018-06-06
AnnouncementXML	Submission_2018-06-06_06:10:36.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008656
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leslie MULLER
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-11 03:34:28	ID requested
⏵ 1	2018-06-06 06:10:38	announced

Muller L, Fornecker L, Chion M, Van Dorsselaer A, Cianf, é, rani S, Rabilloud T, Carapito C, Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics. Sci Rep, 8(1):8260(2018) [pubmed]

curator keyword: Technical

submitter keyword: Tube-Gel, sample preparation, quantitative proteomics

Sarah CIANFERANI
contact affiliation	IPHC (LSMBO - Laboratoire de Spectrométrie de Masse Bio-Organique), UMR 7178, Université de Strasbourg, CNRS
contact email	sarah.cianferani@unistra.fr
lab head
Leslie MULLER
contact affiliation	UMR7178 IPHC-DSA-LSMBO
contact email	leslie.muller@etu.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008656

PRIDE project URI

• PRIDE
  1. PXD008656
     1. Label: PRIDE project
     2. Name: Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics