PXD008644

PXD008644 is an original dataset announced via ProteomeXchange.

Title	The archaeal ATPase PINA interacts with the helicase Hjm via its carboxyl terminal KH domain remodeling and processing replication fork and Holliday junction
Description	PINA is a novel ATPase and DNA helicase highly conserved in archaea, the third domain of life. The PINA from Sulfolobus islandicus (SisPINA) forms a hexameric ring, promotes Holliday junction (HJ) migration, and physically and functionally interacts with Hjc, the HJ specific endonuclease. Here, we show that SisPINA has strong physical interaction with Hjm (Hel308a), a helicase presumably targeting replication forks. In vitro biochemical analysis revealed that Hjm, Hjc, and SisPINA are able to coordinate HJ migration and cleavage in a concerted way. Deletion of the carboxyl 13 amino acid residues abolishes the interaction between SisPINA and Hjm. Crystal structure analysis showed that the carboxyl 70 amino acid residues fold into a type II KH domain which, in other proteins, functions in binding RNA and ssDNA. The KH domain not only mediates the interactions of PINA with Hjm and Hjc but also regulates the hexameric assembly of PINA. Our results collectively suggest possible mechanisms for SisPINA, Hjm, and Hjc to work together in replication fork regression, HJ formation, and HJ cleavage.
HostingRepository	PRIDE
AnnounceDate	2018-10-22
AnnouncementXML	Submission_2018-10-22_09:08:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Green
SpeciesList	scientific name: Sulfolobus islandicus REY15A; NCBI TaxID: 930945;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-10 01:32:41	ID requested
⏵ 1	2018-10-22 09:08:44	announced

Zhai B, DuPrez K, Han X, Yuan Z, Ahmad S, Xu C, Gu L, Ni J, Fan L, Shen Y, The archaeal ATPase PINA interacts with the helicase Hjm via its carboxyl terminal KH domain remodeling and processing replication fork and Holliday junction. Nucleic Acids Res, 46(13):6627-6641(2018) [pubmed]

curator keyword: Biological

submitter keyword: Sulfolobus islandicus, Holliday junction, replication fork, The archaeal ATPase PINA

Yulong Shen
contact affiliation	State Key Laboratory of Microbial Technology, Microbiology and Biotechnology Institute, Shandong University
contact email	yulgshen@sdu.edu.cn
lab head
Rachel Green
contact affiliation	PTM Biolabs Inc.
contact email	xuan_mao@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008644
     1. Label: PRIDE project
     2. Name: The archaeal ATPase PINA interacts with the helicase Hjm via its carboxyl terminal KH domain remodeling and processing replication fork and Holliday junction