PXD008643

PXD008643 is an original dataset announced via ProteomeXchange.

Title	THE EXOPROTEOME PROFILES OF THREE STAPHYLOCOCCUS SAPROPHYTICUS STRAINS REVEAL DIVERSITY IN PROTEIN SECRETION CONTENT
Description	Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors have been characterized, such as urease, autolysins, adhesins, hemagglutinins and cell wall proteins, large-scale proteomic studies have not been performed within this species. In our research, we performed the characterization of the exoproteome from three clinical S. saprophyticus strains (ATCC 15305-capsular strain, 7108 non-capsular strain and 9325, a strain containing a thick capsule) which were cultured until the stationary phase of bacterial growth by using mass spectrometry approach. In these strains we observed a core of 72 secreted proteins. In addition, we identified proteins that were not secreted by all the strains. It was possible to detect diversity in the protein profiles of the exoproteomes, and consequently proteins that were differentially expressed were identified. Interestingly, strain 7108 presented no secretion of three antigenic proteins, including the classical SsaA antigen. In addition, the level of antigenic proteins secreted by strain 9325 was higher than in ATCC 15305. This result was confirmed by Western blot analysis using anti-SsaA polyclonal antibodies, and no production or secretion of SsaA was detected in strain 7108. Moreover, when compared with the other strains that were analyzed, it was possible to detect higher levels of proteases secreted by strain 7108. The results reveal diversity in protein secretion among strains. This research is an important first step towards understanding the variability in S. saprophyticus and could be significant in explaining differences in virulence.
HostingRepository	PRIDE
AnnounceDate	2018-08-29
AnnouncementXML	Submission_2018-10-04_08:58:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CLAYTON BORGES
SpeciesList	scientific name: Staphylococcus saprophyticus; NCBI TaxID: 29385;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-10 01:27:57	ID requested
1	2018-08-29 05:37:59	announced
⏵ 2	2018-10-04 08:58:09	announced	Updated publication reference for PubMed record(s): 30269860.

Oliveira AS, Rosa IIR, Novaes E, Oliveira LS, Baeza LC, Borges CL, Marlinghaus L, Soares CMA, Giambiagi-deMarval M, Parente-Rocha JA, The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents. Microbiol Res, 216():85-96(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Staphylococcus saprophyticus, exoproteome, diversity, SsaA secretion, proteases, antigenic proteins

Juliana Alves Parente Rocha
contact affiliation	Biochemistry and molecular biology department, Universidade Federal de Goias, Brazil
contact email	juparente@gmail.com
lab head
CLAYTON BORGES
contact affiliation	Biochemistry and molecular biology department, Universidade Federal de Goias, Brazil
contact email	clbluiz2@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008643
     1. Label: PRIDE project
     2. Name: THE EXOPROTEOME PROFILES OF THREE STAPHYLOCOCCUS SAPROPHYTICUS STRAINS REVEAL DIVERSITY IN PROTEIN SECRETION CONTENT