PXD008640

PXD008640 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Simultaneous Quantification of Multiple Post-Translational Modifications by 'One-Pot' Affinity Enrichment
Description	Here we use a quantitative label-free proteomics workflow - "one-pot" enrichment - for the comprehensive identification and quantification of lysine acetylated and succinylated peptides from a single sample. Coupled with label-free, data-independent acquisition (DIA), we identified and quantified 2235 acetylated and 2173 succinylated peptides from mouse liver protein. We show peak areas are highly correlated between one-pot and single-antibody enrichments. Finally, we show this method enables the detection of acetylation and succinylation on the same peptide.
HostingRepository	MassIVE
AnnounceDate	2018-01-09
AnnouncementXML	Submission_2018-01-09_10:37:42.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nate
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Acetyl; Succinyl; Oxidation; Gln->pyro-Glu
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-09 10:30:59	ID requested
⏵ 1	2018-01-09 10:37:43	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: acetylation, succinylation, one-pot, post-translational modifications, PTM

submitter keyword: acetylation, succinylation, one-pot, post-translational modifications, PTM

Contact List

Birgit Schilling
contact affiliation	The Buck Institute for Research on Aging
contact email	bschilling@buckinstitute.org
lab head
Nate
contact affiliation	The Buck Institute for Research on Aging
contact email	nbasisty@buckinstitute.org
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081906