PXD008610

PXD008610 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Lactuca sativa L. iTRAQ - Quantitative Proteomics Analysis of Lettuce (Lactuca sativa L
Description	Bolting is a key process in the growth and development of lettuce (Lactuca sativa L.). High temperature can induce earlier bolting which decreases in both quality and production of lettuce. However, knowledge underlying lettuce bolting is still lacking. To better understand the molecular basis of bolting, a comparative proteomics analysis was conducted on lettuce stems in the bolting period induced by high temperature (33 °C) compared with a control (20 °C) using iTRAQ-based proteomics, phenotypic measures, and biological verifications. High temperature induced lettuce bolting, while control temperature did not. Of the 6656 proteins identified, 758 proteins significantly altered their expression level induced by high-temperature relative to the control, of which 409 were up-regulated and 349 down-regulated. Proteins with abundance level change were mainly involved in photosynthesis, carbohydrate metabolism, stress response, hormone synthesis, and signal transduction. These differential proteins were mainly enriched in pathways associated with photosynthesis and tryptophan metabolism involving in auxin (IAA) biosynthesis. Among the differentially expressed proteins associated with photosynthesis and tryptophan metabolism were up-regulated. Moreover, in gibberellin (GA) biosynthesis pathway, 10 of main enzymes of P450 were up-regulated. Proteins related to SAUR and GRP, implicated in IAA and GA signal transduction were up-regulated, and the phosphorylation and ubiquitination related proteins regulating IAA and GA signal transduction were also induced. These findings indicate that a high temperature enhances the function of photosynthesis, IAA and GA synthesis and signal transduction to promote the process of bolting, which is in line with the physiology and transcription levels of IAA and GA metabolism. Our data provide a first comprehensive dataset for gaining novel understanding of the molecular basis underlying lettuce bolting induced by high temperature. It is potentially important for further functional analysis and genetic manipulation for molecular breeding to breed new cultivar of lettuce to restrain early bolting, which is vital for improving vegetable quality.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:20.307.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinghong Hao
SpeciesList	scientific name: Lactuca sativa; NCBI TaxID: 4236;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-08 03:18:47	ID requested
1	2021-03-04 12:42:58	announced
⏵ 2	2024-10-22 04:06:23	announced	2024-10-22: Updated project metadata.

Publication List

Hao JH, Zhang LL, Li PP, Sun YC, Li JK, Qin XX, Wang L, Qi ZY, Xiao S, Han YY, Liu CJ, Fan SX, L.) Reveals Molecular Basis-Associated Auxin and Photosynthesis with Bolting Induced by High Temperature. Int J Mol Sci, 19(10):(2018) [pubmed]
10.3390/ijms19102967;

Hao JH, Zhang LL, Li PP, Sun YC, Li JK, Qin XX, Wang L, Qi ZY, Xiao S, Han YY, Liu CJ, Fan SX, L.) Reveals Molecular Basis-Associated Auxin and Photosynthesis with Bolting Induced by High Temperature. Int J Mol Sci, 19(10):(2018) [pubmed]

10.3390/ijms19102967;

Keyword List

curator keyword: Biological
submitter keyword: high temperature, western-blot, bolting,lettuce, proteome, iTRAQ

curator keyword: Biological

submitter keyword: high temperature, western-blot, bolting,lettuce, proteome, iTRAQ

Contact List

Shuangxi Fan
contact affiliation	Beijing University of Agriculture
contact email	fsx20@163.com
lab head
Jinghong Hao
contact affiliation	Beijing University of Agriculture
contact email	haojinghong2013@126.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD008610

PRIDE project URI

Repository Record List

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