PXD008606

PXD008606 is an original dataset announced via ProteomeXchange.

Title	BCL11B_AP-MS
Description	This study investigates whether the N-terminal CCHC zinc finger motif is necessary and sufficient for the formation of BCL11B dimers, using fluorescence microscopy and Förster resonance energy transfer-assisted fluorescence-activated cell sorting (FACSFRET). The role of the CCHC motif in homodimer assembly was further investigated using affinity purification followed by mass spectrometry (AP-MS). Functional studies investigated the role of dimerization in the induction of cell cycle arrest and protection against DNA damage-induced apoptosis.
HostingRepository	PRIDE
AnnounceDate	2018-01-12
AnnouncementXML	Submission_2018-01-12_05:24:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Praveen Sappa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TSQ Vantage; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-08 00:19:52	ID requested
⏵ 1	2018-01-12 05:24:35	announced

Grabarczyk P, Winkler P, Delin M, Sappa PK, Bekeschus S, Hildebrandt P, Przybylski GK, V, ö, lker U, Hammer E, Schmidt CA, The N-Terminal CCHC Zinc Finger Motif Mediates Homodimerization of Transcription Factor BCL11B. Mol Cell Biol, 38(5):(2018) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

curator keyword: Biological

submitter keyword: AP-MS, Co-immuno precipitation, LC-MS/MS, SRM, PPIN

Christian Schmidt
contact affiliation	komm. Direktor for Innere Medizin mit Schwerpunktbezeichnung Hämatologie und internistische Onkologie, Universitätsmedizin Greifswald.
contact email	caschmid@uni-greifswald.de
lab head
Praveen Sappa
contact affiliation	Universitätsmedizin Greifswald
contact email	praveen.kumar@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD008606

PRIDE project URI

• PRIDE
  1. PXD008606
     1. Label: PRIDE project
     2. Name: BCL11B_AP-MS