PXD008601

PXD008601 is an original dataset announced via ProteomeXchange.

Title	Quantitative metaproteomics of medieval dental calculus reveals individual oral health status
Description	The composition of the ancient oral microbiome has recently become possible to investigate by using advanced biomolecular methods such as metagenomics and metaproteomics. This study presents a look at the individuality of the metaproteomes from 22 medieval Danish dental calculus samples. The proteomics data suggest two distinct groups; a healthy and disease-susceptible. Comparison to modern healthy calculus samples supports this hypothesis. The osteological inspections of the samples does not immediately support the grouping made by proteomics data, making us believe that this will add a new and exciting level of information. We identify 3671 protein-groups across all medieval samples and thus expanding the depth of previous studies more than ten times. As a part of future perspective for further depth in these types of samples we performed offline high pH fractionation in combination with TMT labelling and achieved ~30% more protein identifications and reduced costly mass spectrometry time.
HostingRepository	PRIDE
AnnounceDate	2018-11-26
AnnouncementXML	Submission_2018-11-26_07:29:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rosa Jersie-Christensen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-05 01:55:52	ID requested
⏵ 1	2018-11-26 07:29:48	announced

Jersie-Christensen RR, Lanigan LT, Lyon D, Mackie M, Belstr, ø, m D, Kelstrup CD, Fotakis AK, Willerslev E, Lynnerup N, Jensen LJ, Cappellini E, Olsen JV, Quantitative metaproteomics of medieval dental calculus reveals individual oral health status. Nat Commun, 9(1):4744(2018) [pubmed]

curator keyword: Metaproteomics

submitter keyword: Calculus, Metaproteomics

Jesper Velgaard Olsen
contact affiliation	Jesper Velgaard Olsen, PhD Vice Director, Professor Novo Nordisk Foundation Center for Protein Research Proteomics Program University of Copenhagen Faculty of Health and Medical Sciences
contact email	jesper.olsen@cpr.ku.dk
lab head
Rosa Jersie-Christensen
contact affiliation	University of Copenhagen
contact email	rosa.rjc@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD008601

PRIDE project URI

• PRIDE
  1. PXD008601
     1. Label: PRIDE project
     2. Name: Quantitative metaproteomics of medieval dental calculus reveals individual oral health status