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PXD008597

PXD008597 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGenus wide venom proteomics of cobras (Naja)
DescriptionGenus-wide proteomics analysis of cobra (Naja) venoms. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive) . Spectrum-Protein matching was performed with TopPic1.1 against a genus wide translated transcriptome database and NCBI protein entries from the najas. For bottom-up analysis, HPLC separated fraction of venom were dried down, reduced with triethylphosphine and alkylated with 2-iodoethanol. HPLC-MS/MS experiments were performed on normalflow UHPLC-QTOF system (AB Sciex 5600 TripleTOF). Data analysis (PSM) was performed ProteinPilot version 4.0 and the transcriptome derived protein sequence database.
HostingRepositoryMassIVE
AnnounceDate2022-10-24
AnnouncementXMLSubmission_2022-10-24_10:12:21.450.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniel Petras
SpeciesList scientific name: Naja; NCBI TaxID: 8638;
ModificationListunknown modification
Instrumentinstrument model; TripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-04 17:11:14ID requested
12022-10-24 10:12:23announced
Publication List
Kazandjian TD, Petras D, Robinson SD, van Thiel J, Greene HW, Arbuckle K, Barlow A, Carter DA, Wouters RM, Whiteley G, Wagstaff SC, Arias AS, Albulescu LO, Plettenberg Laing A, Hall C, Heap A, Penrhyn-Lowe S, McCabe CV, Ainsworth S, da Silva RR, Dorrestein PC, Richardson MK, Guti, é, rrez JM, Calvete JJ, Harrison RA, Vetter I, Undheim EAB, W, ü, ster W, Casewell NR, Convergent evolution of pain-inducing defensive venom components in spitting cobras. Science, 371(6527):386-390(2021) [pubmed]
Keyword List
submitter keyword: Top-down Proteomics, Top-down Venomics, Naja, Snake venom, Venom Proteomics, Cobra
Contact List
Eivind Undheim
contact affiliationThe University of Queensland
contact emaile.undheim@uq.edu.au
lab head
Daniel Petras
contact affiliationUniversity of California San Diego
contact emaildpetras@ucsd.edu
lab head
Daniel Petras
contact affiliationUC San Diego
contact emailxsirdanielx@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081885/