PXD008596

PXD008596 is an original dataset announced via ProteomeXchange.

Title	Local and global influences on protein turnover in neurons and glia.
Description	Proteins exhibit dynamics in their expression level in response to intracellular and extracellular signals. Regulation of protein turnover allows cells to rapidly and efficiently remodel their proteomes. It is known that proteins can show very different turnover rates in different tissue of the same organism, but little is known about protein turnover rates in different brain cell types. We used a dynamic SILAC approach to determine protein half-lives in primary hippocampal cultures (containing a mixture of neurons and glia cells) as well as in neuron-enriched and glia-enriched cultures. We determined the protein half-lives for over 5100 proteins and found half-lives ranging from <1 to > 20 days with a median half-life of 5.4 days in mixed cultures. Membrane proteins as a group were shorter-lived and mitochondrial proteins, surprisingly, were longer-lived. Proteins in glia possessed significantly shorter half-lives than the same proteins in neurons. The presence of glia sped up or slowed down the half-lives of neuronal proteins. Our results demonstrate that both the cell-type of origin as well as the nature of the extracellular environment have potent influences on protein turnover.
HostingRepository	PRIDE
AnnounceDate	2018-06-05
AnnouncementXML	Submission_2018-06-24_23:52:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-04 04:06:31	ID requested
1	2018-06-05 08:14:26	announced
⏵ 2	2018-06-24 23:52:27	announced	Updated publication reference for PubMed record(s): 29914620.

D, ö, rrbaum AR, Kochen L, Langer JD, Schuman EM, Local and global influences on protein turnover in neurons and glia. Elife, 7():(2018) [pubmed]

curator keyword: Biological

submitter keyword: Proteome turnover, SILAC, neurons, glia

Julian David Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008596
     1. Label: PRIDE project
     2. Name: Local and global influences on protein turnover in neurons and glia.