PXD008592

PXD008592 is an original dataset announced via ProteomeXchange.

Title	Identification of Clostdridium difficile Immunoreactive Spore Proteins of the Epidemic strain R20291
Description	Clostridium difficile infections are the leading cause of diarrhea associated to the use of antibiotics. During infection, C. difficile initiates a sporulation cycle leading to the persistence of C. difficile spores in the host and disease dissemination. Nowadays, the development of vaccine and passive immunization therapies against C. difficile have focused on toxins A and B. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the outer layers of C. difficile spores as potential candidates for the development of immunotherapy and/or diagnostic methods against this devastating infection. Experimental design. To identify potential immunogenic proteins on the surface of C. difficile R20291, spore coat/exosporium extracts were separated by two-dimensional electrophoresis (2-DE) and analyzed for reactivity against C. difficile spore-specific goat sera. Finally, the proteins present at the spot of the interest were in-gel digested with chymotrypsin, and the peptides generated were separated by nanoUPLC followed by MS/MS using a Quad-TOF MS, and corroborated by Ultimate 3000RS nano UHPLC coupled to QExactive Plus Orbitrap MS.
HostingRepository	PRIDE
AnnounceDate	2018-07-05
AnnouncementXML	Submission_2018-07-05_10:25:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008592
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eduardo Callegari
SpeciesList	scientific name: Peptoclostridium difficile (strain R20291) (Clostridium difficile); NCBI TaxID: 645463;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q Exactive; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-03 08:48:46	ID requested
⏵ 1	2018-07-05 10:25:39	announced

Pizarro-Guajardo M, Ravanal MC, Paez MD, Callegari E, Paredes-Sabja D, Identification of Clostridium difficile Immunoreactive Spore Proteins of the Epidemic Strain R20291. Proteomics Clin Appl, 12(5):e1700182(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Clostridium difficile, Spores, Immunoblotting, two-dimensional electrophoresis, CDI patients, Serologic reactivity, isoelectric focusing

Daniel Paredes-Sabja
contact affiliation	Microbiota-Host Interaction & Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile
contact email	daniel.paredes.sabja@gmail.com
lab head
Eduardo Callegari
contact affiliation	University of South Dakota
contact email	Eduardo.Callegari@usd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008592
     1. Label: PRIDE project
     2. Name: Identification of Clostdridium difficile Immunoreactive Spore Proteins of the Epidemic strain R20291