PXD008580

PXD008580 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based quantitative phosphoproteomic analysis of a prp4ka mutant in Arabidopsis thaliana
Description	We identified PRP4 kinase-A (PRP4ka) in a forward genetic screen based on an alternatively-spliced GFP reporter gene in Arabidopsis thaliana (Arabidopsis). Prp4 kinase, which was the first spliceosome-associated kinase shown to regulate splicing in fungi and mammals, has not yet been studied in plants. Analysis of RNA-seq data from the prp4ka mutant revealed widespread perturbations in alternative splicing. A quantitative iTRAQ-based phosphoproteomics investigation of the mutant identified phosphorylation changes in several serine/arginine-rich proteins, which regulate constitutive and alternative splicing, as well as other splicing-related factors. The results demonstrate the importance of PRP4ka in alternative splicing and suggest that PRP4ka may influence alternative splicing patterns by phosphorylating a subset of splicing regulators.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_09:04:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuan-Nan Wen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-02 06:47:24	ID requested
⏵ 1	2018-10-25 09:04:53	announced

Kanno T, Venhuizen P, Wen TN, Lin WD, Chiou P, Kalyna M, Matzke AJM, Matzke M, . Genetics, 210(4):1267-1285(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis thaliana, iTRAQ, quantitative phosphoproteomics, pre-mRNA splicing, prp4ka

Antonius Matzke
contact affiliation	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
contact email	antoniusmatzke@gate.sinica.edu.tw
lab head
Tuan-Nan Wen
contact affiliation	Institute of Plant and Microbial Biology, Academia Sinica, Taiwan
contact email	tnwen@sinica.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008580
     1. Label: PRIDE project
     2. Name: iTRAQ-based quantitative phosphoproteomic analysis of a prp4ka mutant in Arabidopsis thaliana