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PXD008579

PXD008579 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein and peptide turnover measurements using pulsed SILAC-TMT approach
DescriptionWe evaluated the feasibility of a workflow combining dynamic SILAC experiments with tandem mass tag (TMT)-labeling of ten pulse time-points. Replicate analysis established that the same reproducibility of turnover rates can be obtained for peptides as for proteins facilitating proteoform resolved investigation of protein stability.
HostingRepositoryPRIDE
AnnounceDate2018-02-09
AnnouncementXMLSubmission_2018-02-09_10:45:57.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJana Zecha
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-02 06:37:04ID requested
12018-02-09 10:45:58announced
Publication List
Zecha J, Meng C, Zolg DP, Samaras P, Wilhelm M, Kuster B, Peptide Level Turnover Measurements Enable the Study of Proteoform Dynamics. Mol Cell Proteomics, 17(5):974-992(2018) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: protein turnover, pulsed SILAC, TMT, synthesis rates, degradation rates, proteoforms, splice variants, posttranslational cleavage, posttranslational modification
Contact List
Bernhard Kuster
contact affiliationChair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany
contact emailkuster@tum.de
lab head
Jana Zecha
contact affiliationTUM
contact emailjana.zecha@tum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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