PXD008554

PXD008554 is an original dataset announced via ProteomeXchange.

Title	Quantitative secretomics during cardiomyogenic differentiation of human pluripotent stem cells
Description	Cardiovascular diseases are a major cause of life-threatening burden around the world. The heart has a very low regeneration capacity and donor organs for transplantation are scarce. Therefore regeneration of lost myocardium with stem cell-derived cardiomyocytes (CMs) provides an attractive strategy for heart repair. Human pluripotent stem cells (hPSCs) can be efficiently differentiated in vitro into CMs but the molecular mechanisms behind this process are still not fully understood. In particular identification of secreted autocrine and/or paracrine factors that function as important extrinsic signals remained elusive because the mass spectrometry (MS)-based identification of secreted proteins from cell culture supernatants is impeded by high levels of albumin present in common differentiation media. Thus we established an albumin-free cardiomyogenic differentiation medium and performed secretomics at seven different time points during in vitro differentiation. This analysis led to the identification of 4832 proteins with 1802 being significantly altered during differentiation and 431 of these were annotated as secreted according to gene ontology. Bioinformatics revealed enrichment of extrinsic Wnt pathway-related proteins 3 days upon induction of differentiation and of extracellular matrix proteins in the resulting CMs. Numerous extrinsic components of Wnt, Activin A, Nodal, TGFβ, BMP or FGF signaling pathways were quantitatively assessed during differentiation. Notably, the abundance of pathway agonists was generally lower compared to the respective antagonists but their curves of progression over timer were rather similar. We hypothesize that Activin A, Nodal and TGFβ signaling are turned down shortly upon initiation of cardiac differentiation whereas BMP signaling is switched on. Wnt and FGF signaling peaks between d0 and d3 of differentiation and interestingly, Activin A and TGFβ signaling seem to be reactivated at the cardiac progenitor stages and/or in early CMs.
HostingRepository	PRIDE
AnnounceDate	2018-06-20
AnnouncementXML	Submission_2018-06-20_00:19:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Falk Büttner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-22 06:48:42	ID requested
⏵ 1	2018-06-20 00:19:28	announced

Wolling H, Konze SA, H, ö, fer A, Erdmann J, Pich A, Zweigerdt R, Buettner FFR, Quantitative Secretomics Reveals Extrinsic Signals Involved in Human Pluripotent Stem Cell Cardiomyogenesis. Proteomics, 18(14):e1800102(2018) [pubmed]

curator keyword: Biological

submitter keyword: Secretomics, LC-MS/MS, hiPSCs, hESCs, Cardiomyogenesis

Falk Fritz Buettner
contact affiliation	Institute of Clinical Biochemistry Hannover Medical school 30625 Hannover, Germany
contact email	buettner.falk@mh-hannover.de
lab head
Falk Büttner
contact affiliation	Hannover Medical School
contact email	buettner.falk@mh-hannover.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008554
     1. Label: PRIDE project
     2. Name: Quantitative secretomics during cardiomyogenic differentiation of human pluripotent stem cells