PXD008550

PXD008550 is an original dataset announced via ProteomeXchange.

Title	An integrated workflow for cross-linking/mass spectrometry
Description	We present a concise workflow to enhance the mass spectrometric detection of cross-linked peptides by introducing sequential digestion and the database search software Xi. We use sequential digestion to reduce the peptide size and increase identification rates. The methodology is independent of samples complexity, cross-linker choice, method acquisition and can be used with multiple digestion steps.
HostingRepository	PRIDE
AnnounceDate	2019-08-27
AnnouncementXML	Submission_2019-08-27_02:09:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Mendes
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Chaetomium thermophilum; NCBI TaxID: 209285; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-22 03:44:54	ID requested
⏵ 1	2019-08-27 02:09:51	announced

Dataset with its publication pending

curator keyword: Technical

submitter keyword: cross-linking mass spectrometry, sequential digestion, Xi

Juri Rappsilber
contact affiliation	Institute of Biotechnology Technische Universität Berlin Gustav-Meyer-Allee 25 13355 Berlin
contact email	juri.rappsilber@tu-berlin.de
lab head
Marta Mendes
contact affiliation	Department of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany
contact email	m.mendes@tu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD008550

PRIDE project URI

• PRIDE
  1. PXD008550
     1. Label: PRIDE project
     2. Name: An integrated workflow for cross-linking/mass spectrometry