PXD008546 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In vivo DDR1 signalling |
Description | The clinical management of metastatic colorectal cancer (mCRC) faces major challenges. For instance, patients harbouring oncogenic mutations in RAS signalling do not respond to anti-EGFR targeted treatment. Therefore, RAS-independent therapies are needed. Interestingly nilotinib, a clinically approved drug for chronic myeloid leukaemia, inhibits human CRC cell invasion in vitro and reduces their metastatic potential in intrasplenic tumour mouse models. Nilotinib acts by inhibiting the kinase activity of DDR1, a receptor tyrosine kinase for collagens, which we identified as a RAS-independent inducer of CRC metastasis. Using quantitative phosphoproteomics, we identified BCR as a new DDR1 substrate and demonstrated that nilotinib prevents DDR1-mediated BCR phosphorylation on Tyr177, which is important for maintaining -catenin transcriptional activity necessary for tumour cell invasion. DDR1 kinase inhibition also reduced the invasion of patient-derived metastatic and circulating CRC cell lines. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-31 |
AnnouncementXML | Submission_2018-02-16_05:49:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Debora Bonenfant |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-12-22 02:09:19 | ID requested | |
1 | 2018-01-31 00:53:14 | announced | |
⏵ 2 | 2018-02-16 05:49:07 | announced | Updated publication reference for PubMed record(s): 29438985. |
Publication List
Jeitany M, Leroy C, Tosti P, Lafitte M, Le Guet J, Simon V, Bonenfant D, Robert B, Grillet F, Mollevi C, El Messaoudi S, Otandault A, Canterel-Thouennon L, Busson M, Thierry AR, Martineau P, Pannequin J, Roche S, Sirvent A, new therapeutic strategy for metastatic colorectal cancer. EMBO Mol Med, 10(4):(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: metastatic colorectal cancer, signalling, DDR1 tyrosine kinase, |
Contact List
Debora Bonenfant, Novartis Institutes for BioMedical Research |
contact affiliation | Debora Bonenfant, Novartis Institutes for BioMedical Research, Basel, Switzerland |
contact email | debora.bonenfant@novartis.com |
lab head | |
Debora Bonenfant |
contact affiliation | Novartis Institutes for BioMedical Research |
contact email | debora.bonenfant@novartis.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD008546 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008546
- Label: PRIDE project
- Name: In vivo DDR1 signalling