PXD008545

PXD008545 is an original dataset announced via ProteomeXchange.

Title	Proteomics of human cord blood reticulocyte-derived exosomes
Description	Human reticulocytes were enriched from human umbilical blood, and cultured in vitro for the production of reticulocyte-derived exosomes (HuRex)to unveil their proteomic composition. As research on the molecular-cargo of exosomes is largely confounded by the lack of a “gold-standard” methodology, we applied several approaches for obtaining HuRex prior to MS. HuRex preparations were obtained from cultures in absence of serum (AS) from three donors and isolated by means of size-exclusion chromatography (SEC) (n=3) and ultracentrifugation (UC) (n=3). HuRex preparations in the presence of serum were obtained from three other donors and purified by SEC (n=3) and UC (n=1).
HostingRepository	PRIDE
AnnounceDate	2018-09-24
AnnouncementXML	Submission_2018-09-24_03:39:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hernando A del Portillo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-22 02:03:23	ID requested
⏵ 1	2018-09-24 03:39:39	announced

D, í, az-Varela M, de Menezes-Neto A, Perez-Zsolt D, G, á, mez-Valero A, Segu, í, -Barber J, Izquierdo-Useros N, Martinez-Picado J, Fern, á, ndez-Becerra C, Del Portillo HA, Proteomics study of human cord blood reticulocyte-derived exosomes. Sci Rep, 8(1):14046(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, reticulocyte, exosome, LC-MSMS

Hernando A. del Portillo
contact affiliation	ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain IGTP Institut d’Investigació Germans Trias i Pujol, Badalona, Spain
contact email	hernandoa.delportillo@isglobal.org
lab head
Hernando A del Portillo
contact affiliation	ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain IGTP Institut d’Investigació Germans Trias i Pujol, Badalona, Spain
contact email	hernandoa.delportillo@isglobal.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD008545

PRIDE project URI

• PRIDE
  1. PXD008545
     1. Label: PRIDE project
     2. Name: Proteomics of human cord blood reticulocyte-derived exosomes