PXD008542

PXD008542 is an original dataset announced via ProteomeXchange.

Title	Dissection of affinity captured LINE-1 macromolecular complexes
Description	Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNA-containing RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:13:59.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilya Altukhov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-22 00:33:47	ID requested
1	2018-01-17 23:49:51	announced
⏵ 2	2024-10-22 04:14:01	announced	2024-10-22: Updated project metadata.

Taylor MS, Altukhov I, Molloy KR, Mita P, Jiang H, Adney EM, Wudzinska A, Badri S, Ischenko D, Eng G, Burns KH, Feny, ö D, Chait BT, Alexeev D, Rout MP, Boeke JD, LaCava J, Dissection of affinity captured LINE-1 macromolecular complexes. Elife, 7():(2018) [pubmed]

10.7554/elife.30094;

curator keyword: Biological

submitter keyword: LINE-1, protein interactions, affinity proteomics, L1, mass spectrometry, biochemistry, retrotransposon, ribonucleoprotein, RNP, interactome

John Paul LaCava
contact affiliation	Research Assistant Professor Laboratory of Cellular and Structural Biology The Rockefeller University, USA
contact email	jlacava@mail.rockefeller.edu
lab head
Ilya Altukhov
contact affiliation	Research Institute of Physical Chemical Medicine
contact email	ilya.altukhov@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD008542

PRIDE project URI

• PRIDE
  1. PXD008542
     1. Label: PRIDE project
     2. Name: Dissection of affinity captured LINE-1 macromolecular complexes