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PXD008542

PXD008542 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDissection of affinity captured LINE-1 macromolecular complexes
DescriptionLong Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNA-containing RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.
HostingRepositoryPRIDE
AnnounceDate2018-01-18
AnnouncementXMLSubmission_2018-01-17_23:49:50.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlya Altukhov
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-12-22 00:33:47ID requested
12018-01-17 23:49:51announced
Publication List
Taylor MS, Altukhov I, Molloy KR, Mita P, Jiang H, Adney EM, Wudzinska A, Badri S, Ischenko D, Eng G, Burns KH, Fenyƶ D, Chait BT, Alexeev D, Rout MP, Boeke JD, LaCava J, Dissection of affinity captured LINE-1 macromolecular complexes. Elife, 7():(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: LINE-1, L1, retrotransposon, ribonucleoprotein, RNP, interactome, mass spectrometry, affinity proteomics, biochemistry, protein interactions
Contact List
John Paul LaCava
contact affiliationResearch Assistant Professor Laboratory of Cellular and Structural Biology The Rockefeller University, USA
contact emailjlacava@mail.rockefeller.edu
lab head
Ilya Altukhov
contact affiliationResearch Institute of Physical Chemical Medicine
contact emaililya.altukhov@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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