PXD008536

PXD008536 is an original dataset announced via ProteomeXchange.

Title	Systematic Investigation of Targeted Analysis of Metabolites and Proteins in Tear Fluid
Description	Since the sampling of tear fluid seems rather variable we sought to evaluate a protocol for combined metabolomic and proteomic analyses with regard to clinical applicability. Tear fluid was taken from twelve human volunteers at different time points using specially designed Schirmer strips. Following liquid extraction of metabolites from standardized punchesthe remaining material was processed for bottom-up proteomics. The targeted proteomic analysis of 15 tear proteins revealed a mean intra-individual variation of 23 % merely for the three most abundant proteins. Overall, our newly established workflow opens perspectives for clinical and routine diagnostic applications using well accessible tear fluid.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_09:37:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janina D'Alvise
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-21 08:07:56	ID requested
⏵ 1	2019-11-12 09:37:46	announced

Dammeier S, Martus P, Klose F, Seid M, Bosch D, D'Alvise J, Ziemssen F, Dimopoulos S, Ueffing M, Combined Targeted Analysis of Metabolites and Proteins in Tear Fluid With Regard to Clinical Applications. Transl Vis Sci Technol, 7(6):22(2018) [pubmed]

curator keyword: Technical

submitter keyword: Tear fluid

Sascha Dammeier
contact affiliation	Institute for Ophthalmic Research, Core Facility for Medical Bioanalytics, University of Tübingen, Elfriede-Aulhorn-Strasse 7, 72076 Tübingen, Germany
contact email	sascha.dammeier@uni-tuebingen.de
lab head
Janina D'Alvise
contact affiliation	University of Tuebingen
contact email	janina.dalvise@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD008536

PRIDE project URI

• PRIDE
  1. PXD008536
     1. Label: PRIDE project
     2. Name: Systematic Investigation of Targeted Analysis of Metabolites and Proteins in Tear Fluid