PXD008533

PXD008533 is an original dataset announced via ProteomeXchange.

Title	Defining targets of the bacterial ADP-ribosyltransferase EspJ
Description	Tyrosine phosphorylation is key for signal transduction from exogeneous stimuli, including the defence against pathogenic microbes. Pathogens have acquired mechanisms to subvert protein phosphorylation as a means to control host immune responses and facilitate invasion and dissemination. The bacterial effector protein EspJ is injected into host cells by pathogenic strains of Escherichia coli, Salmonella and Citrobacter rodentium where it can prevent phagocytosis via the inhibitory amidation and ADP ribosylation of Src kinase E310. Here, we found that EspJ can ADP ribosylate members of the Src, Abl, Csk, Tec and Syk kinase families. ADP ribosylation of Csk inhibits its kinase activity, giving EspJ ultimate control over the Src/Csk signalling axis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:13:31.731.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominic Pollard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-21 05:39:28	ID requested
1	2018-03-16 02:29:30	announced
2	2018-04-25 06:32:35	announced	Updated publication reference for PubMed record(s): 29636436.
⏵ 3	2024-10-22 04:13:33	announced	2024-10-22: Updated project metadata.

10.1128/mbio.00170-18;

Pollard DJ, Berger CN, So EC, Yu L, Hadavizadeh K, Jennings P, Tate EW, Choudhary JS, Frankel G, Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ. mBio, 9(2):(2018) [pubmed]

curator keyword: Biological

submitter keyword: EHEC, Human,ADP-ribosyltransferase, LC-MS/MS

Gad Frankel
contact affiliation	Center for Molecular Bacteriology and Infection, Imperial College London
contact email	g.frankel@imperial.ac.uk
lab head
Dominic Pollard
contact affiliation	Imperial College London
contact email	d.pollard13@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008533

PRIDE project URI

• PRIDE
  1. PXD008533
     1. Label: PRIDE project
     2. Name: Defining targets of the bacterial ADP-ribosyltransferase EspJ