PXD008531

PXD008531 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice
Description	Mutations in leucine-rich repeat kinase 2 (LRRK2) segregate with familial Parkinson’s disease (PD) and genetic variation in LRRK2 contributes to risk of sporadic disease. Although knockout of LRRK2 or knock-in of pathogenic mutations into the mouse germline does not result in a PD phenotype, several defects have been reported in the kidneys of LRRK2 knockout mice. To understand LRRK2 function in vivo, we used an unbiased approach to determine which protein pathways are affected in LRRK2 knockout kidneys. We performed two iTRAQ screens on cytosol- and microsome-enriched fractions from 12-month old LRRK2-KO and G2019S knockin kidneys compared to wild type controls. We nominated changes in cytoskeletal-associated proteins, lysosomal proteases, proteins involved in vesicular trafficking and in control of protein translation in LRRK2-KO. Changes were not seen in mice expressing the pathogenic G2019S LRRK2 mutant.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_09:43:30.382.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008531
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jinhui Ding
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Methylthio; Oxidation; iTRAQ8plex
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-21 03:24:52	ID requested
⏵ 1	2022-03-01 09:43:31	announced

Pellegrini L, Hauser DN, Li Y, Mamais A, Beilina A, Kumaran R, Wetzel A, Nixon-Abell J, Heaton G, Rudenko I, Alkaslasi M, Ivanina N, Melrose HL, Cookson MR, Harvey K, Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice. Hum Mol Genet, 27(18):3257-3271(2018) [pubmed]

curator keyword: Biological

submitter keyword: Parkinson’s disease, LRRK2, genetic mouse models, proteomics, membrane trafficking, protein translation, lysosomal function

Mark R Cookson
contact affiliation	Laboratory of Neurogenetics, National Institute on Aging, National Institute of Health, Building 35, Room 1A116 35 Convent Drive Bethesda, MD 20814, USA
contact email	cookson@mail.nih.gov
lab head
Jinhui Ding
contact affiliation	NIH
contact email	dingj@mail.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008531
     1. Label: PRIDE project
     2. Name: Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice